Unconjugated protein

The conjugate may be purified to remove unconjugated protein using gel filtration on a column of resin having a molecular weight cut-off able to accommodate the proteins being separated. 

Remove unconjugated protein and excess cyanoborohydride by gel filtration using a column of Sephadex G-50 or G-75. 

Use unconjugated protein A (36 pg/mL in SC) to block protein A binding sites on the Fc portion of rabbit anti-sheep. 

While proteins are synthesized on ribosomes the translation product is often subject to considerable post-translational modification that can involve proteolytic processing of the initial proprotein and covalent modification of the processed protein by glycosylation (addition of sugar residues), acylation (e.g. with fatty acids), methylation and phosphorylation. Unconjugated proteins are those in which there is no non-amino acid substituent and conjugated proteins are those that have been modified with non-amino acid entities. 

Quantitative precipitation tests indicate both how much hapten-specific antibody is present and how much unconjugated protein would be required to absorb out all the antibodies directed against the protein alone. This absorption can then be done by incubating equivalence concentrations of antigen and serum and removing the precipitate. Absorption can also be done with protein-agarose affinity columns. In addition, the antibody can be purified with hapten-bearing affinity columns, from which hapten-specific antibody can be eluted with 2 M acetic acid or with excess hapten. 

Prolamines a group of simple (unconjugated) proteins, soluble in 90% ethanol. They occur in cereals, and contain up to 15 % proline and 30-45 % glutamic add, but they have only low contents of essential amino acids. The chief representatives are gliadin (wheat and rye), zein (maize contains no tryptophan or lysine) and hordein (barley contains no lysine). Oats and rice do not contain P. 

On the basis of electron microscopic studies, Robertson (1967) has modified the Danielli-Davson model, presenting a new model—the unit membrane hypothesis—for all cell membranes. This model was similar to that of Danielli-Davson but with a few significant changes. The number of bimolecular phospholipid layers in Robertson s model was restricted to one, and the membrane was considered to be asymmetrical, with mucopolysaccharide or mucoprotein on the outside and unconjugated protein on the inner part of the membrane (Fig. 2). The... 

Continue to wash the gel with 0.15M NaCl (saline solution) until the absorbance at 280nm is down to baseline. The protein eluting from the column at this point is unconjugated enzyme. 

This antibody is raised against the protein-based CD3 antigen, present on the cell surface of most T-lymphocytes. I.v. administration of (unconjugated) antibody appears to block normal functioning of such T-cells and promote their clearance from the blood. However, upon cessation of antibody administration, CD3 positive cell numbers rapidly revert to normal values. Therefore, maintenance immunosuppressives (e.g. with cyclosporine) must subsequently be restored. 

Unconjugated bilirubin is taken into the hepatocytes by binding to membrane transport proteins and transported through the liver cells to the SER by proteins called ligandins. The SER is the location of a specific bilirubin-UDP-glucuronosyl transferase... 

---