Ribonuclease performic acid-oxidized

E. Haas, G. T. Montelione, C. A. McWherter, and H. A. Scheraga, Local structure in a tryptic fragment of performic acid oxidized ribonuclease A corresponding to a proposed polypeptide chain-folding initiation site detected by tyrosine fluorescence lifetime and proton magnetic resonance measurements, Biochemistry 26, 1672-1683 (1987). 

Bigelow (1960) has found that all six of the phenolic groups of performic acid-oxidized ribonuclease behave normally, which is to be expected since the oxidized protein is believed to be highly unfolded. More interesting is his finding that a pepsin-inactivated preparation of ribonuclease can be prepared which contains five normal phenolic groups and one buried one. 

Figure 16. Hydrolysis rate of bovine ribonuclease relative to that of performic acid-oxidized ribonuclease as a function of temperature (155). The follotving enzymes were used O, aminopeptidase , carboxy-peptidase A A, trypsin and , chymotrypsin.

Analogous results have been obtained with reduced-carboxymethyl-ated bovine serum albumin, performic acid-oxidized ribonuclease, re-duced-carboxymethylated lysozyme, and several other proteins. 

Ribonuclease, oval- Performic acid oxidation nor- Tramor and Shugar... 

Insulin consists of two different polypeptide chains held together by —S—S— bridges. They can be separated by oxidation with performic acid. Similarly, ribonuclease consists of 124 amino acids and —S—S— bridges which can also be separated. 

Figure 6. Chromatograms illustrating time course of oxidation of ribonuclease A by performic acid. Experimental conditions were similar to those described under...

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