Truncated genes

Misawa, N. et al.. Expression of a tomato cDNA coding for phytoene synthase in Escherichia coli, phytoene formation in vivo and in vitro, and functional analysis of the various truncated gene products, J. Biochem. (Tokyo) 116, 980, 1994. 

Mikhitarian K, Reott S, Hoover L, et al. Enhanced detection of RNA from paraffin-embedded tissue using a panel of truncated gene-specific primers for reverse transcription. BioTechniques 2004 36 474 178. 

Brisson N. Verma, D.P.S. (1982). Soybean leghemoglobin gene family Normal, pseudo, and truncated genes. Proceedings of the National Academy of Sciences (USA) 79, 4055-9. 

Many crystallization reports emphasize the need to use pure proteins to ensure crystal reproducibility. The application of recombinant DNA technology to the production of truncated gene products promises to alleviate many of the difficulties associated with purifying protein fragments produced with proteolytic enzymes. 

The number of nonintact genes, containing truncated genes and pseudogenes, is shown in parentheses. 

JM Gettemy, D Li, M Alic, MH Gold. Truncated gene reported system for smdying the regulation of manganese peroxidase. Curr Genet 31 519-524, 1998. 

Real time for a panel of truncated gene-specific primers... 

Truncated Forms. Tmncated forms of hGH have been produced, either through the actions of enzymes or by genetic methods. 2-CAP, generated by the controlled actions of the trypsin, has the first eight residues at the N-terminus of hGH removed. Other tmncated versions of hGH have been produced by modification of the gene before expression in a suitable host. The first 13 residues have been removed to yield a derivative having distinctive biological properties (30). In this latter case the polypeptide chain is not cleaved. 

On the other hand, EFN-a may also be involved in the activation of autoreactive T-cells as has been proposed for type I diabetes. An DFN-a inducible superantigen, encoded by the truncated envelope gene of a human endogenous retrovirus and specifically activating V 37 T-cells, has been detected in pancreatic lesions from type I diabetes patients, infiltrated by V 37 T-cells. Since IFN-a expression could be detected in pancreatic (3 cells in conceit with persistent viral infections, there is a clear link between viral infections and autoimmunity via IFN-a-stimulated superantigen expression. 

A naturally occurring animal model of PFK-M deficiency has been reported in English springer spaniels. Molecular analysis of this canine PFK-M deficiency disclosed that the enzyme deficiency was caused by a nonsense mutation in the penultimate exon of the PFK-M gene, leading to rapid degradation of a truncated (40 amino acid residues) and therefore unstable enzyme protein (SI8). 

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