Tropomyosin properties

Ishi, Y., and Lehrer, S.S. (1986) Effects of the state of the succinimido-ring on the fluorescence and structural properties of pyrene maleimide-labeled an-tropomyosin. Biophys./. 50, 75-80. 

Tropomyosin is a protein found in vertebrates and invertebrates, but only the protein from invertebrates has allergic properties. It is frequently found in sea foods, such as shrimps, crabs, American lobsters. Pacific flying squids, and also in some species of cockroaches (Blattela germanica, Periplaneta americand), moths, spiders, and house dust mites (Dermatophagoides pteronyssinus, Dermatophagoides farinae) (Witteman et ah, 1994). 

Asturias, J. A., Eraso, E., Arilla, M. C., Gomez-Bayon, N., Inacio, E., and Martinez, A. (2002). Cloning, isolation, and IgE-binding properties of Helix aspersa (brown garden snail) tropomyosin. Int. Arch. Allergy Immunol. 128, 90-96. 

Historically, coiled coils were identified with long fibrous molecules, from which their structural properties had been determined. Fiber diffraction studies on proteins of the k-m-e-f class were highly successful, initially on dried specimens but later also on native samples (Cohen and Holmes, 1963). However, these proteins turned out to be very difficult to analyze by high-resolution X-ray crystallography for the same reasons that made them so amenable to fiber diffraction—their tendency to aggregate into fibers rather than crystals and the extreme dimensions of their asymmetric units. It took decades to obtain a working structure for tropomyosin [at 15 A resolution (Phillips et al., 1986) at 9 A (Whitby et al.,... 

Repeating surface residues of tropomyosin appear to interact direcdy with recognition sites on consecutive monomers of F-actin. We first discuss, however, the repeat in the core, which, although less regular than that of certain surface residues (see below), has a clearly understood effect on the conformational and dynamic properties of the coiled-coil. 

Heeley, D. H., Watson, M. H., Mak, A. S., Dubord, P., and Smillie, L. B. (1989). Effect of phosphorylation on the interaction and functional properties of rabbit striated muscle alpha-tropomyosin. / Biol. Chem. 264, 2424-2430. 

Maytum, R., Geeves, M. A., and Konrad, M. (2000). Actomyosin regulatory properties of yeast tropomyosin are dependent upon N-terminal modification. Biochemistry 39, 11913-11920. 

Perry, S. V. (2001). Vertebrate tropomyosin Distribution, properties and function. 

Phillips, G. N., and Chacko, S. (1996). Mechanical properties of tropomyosin and implications for muscle regulation. Biopolymers 38, 89-95. 

Sano, K.-I., Maeda, K., Oda, T., and Maeda, Y. (2000). The effect of single residue substitutions of serine-283 on the strength of head-to-tail interaction and actin binding properties of rabbit skeletal muscle a-tropomyosin. /. Biochem. 127, 1095-1102. 

After the war, Bailey returned to studies on muscle, and announced in 1946 the isolation from vertebrate muscle of tropomyosin B, a new myofibrillar protein with unusual properties. This achievement and the later discovery of the family of tropomyosins A are Bailey s most important contributions to the muscle field. The latter protein was shown to be responsible for the paramyosin X-ray diffraction pattern characteristic of molluscan adductor muscles and opened up a new field of interest. The tropomyosins possess a number of remarkable properties which are just those which we would have expected to excite his interest—one cannot help feeling that these proteins were almost custom-built for him. Their function is still something of a mystery but the... 

This section represents an extension of our previous reviews (Ebashi and Endo, 1968 Ebashi et al, 1969 Ebashi, 1974a, 1980) and concerns mainly the interacting properties and arrangement of the three troponin components and tropomyosin in reference to calcium regulation. The protein source is rabbit skeletal muscle unless otherwise mentioned. 

In this section, properties of calcium regulatory proteins from vertebrate skeletal muscle were reviewed with particular reference to the physiological structure and function that have been the major interest since the discovery of native tropomyosin. 

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