Triterpenoid fractions isolation

Table 4. Effects of triterpenoid fractions, and acidic and neutral fractions, isolated from triterpenoid fractions of Ganoderma lucidum, on angiogenesis induced by MalrigeK supplemented with VEGF and heaprin1...

Furthermore, the isolation of the active substancc(s) from the acidic fraction of the triterpenoid fractions, was performed using the Matrigel-implanted angiogenesis assay, and seven fractions were obtained from the acidic fraction by silica gel column chromatography. 

The methods for isolation of steroid saponins are similar to those of triterpenoid saponins. Since glycosides, as a class, are particularly prone to enzymatic or microbial degradation, processing of plant material needs to be started soon after collection to avoid delays. Air-dried powdered plant material is defatted and then extracted, either with cold or hot methanol or ethanol or with 50% aqueous ethanol or methanol at ambient temperature. Usually the extract is concentrated at reduced pressure, macerated with water, and partitioned successively using ethyl acetate and re-BuOH. Most of the saponin constituents are found in the n-BuOH soluble fraction. However, highly polar glycosides may be found in the aqueous layer. 

It should be noted that while these compounds show potent in vivo antileukemic activity against P-388 leukemia in mice, very similar compounds, e g., roridin D, Fig. (61) show no in vivo activity. The key difference seems to be the presence of an oxygen substituent in the ring A of the trichothecene nucleus in the baccharinoids. More recently, screening of plants from South America for antineoplastic activity and subsequent assay-guided fractionation resulted in the isolation of several pentacyclic triterpenoids as active constituents from some Baccharis spp [85],... 

The MeOH extract of the freshly collected whole plant of S. officinalis was suspended in water and partitioned successively with EtOAc and n-BuOH. The aqueous part, on chromatography over Diaion HP-20 followed by repeated MPLC and HPLC purification afforded two major triterpenoid saponins, saponariosides A and B. Similarly, the -BuOH soluble fraction afforded six triterpenoid saponins designated as saponariosides C-H. Investigation of the plant material collected from different geographical locations led to the isolation of previously reported saponariosides C, E, F and G along with five more new saponins, saponariosides I-M (Fig. 7), from which two new sapogenins have been characterized as VIII and XII (Fig. 1). However, saponarioside D, the major constituent of the -BuOH soluble fraction as reported previously by us [21], could not be detected. 

As an extension of chemical investigation on the pharmaceutically important naturally occurring saponins [48-57], Mahato and his coworkers isolated three acylated triterpenoid bisglycosides, acaciasides A (25), B (26) and C (27) from the water soluble saponin fraction obtained... 

In addition to H and NMR analysis, TLC coupled in an offline manner with electrospray mass spectrometry (ESI-MS) and high-resolution ESI Fourier transform ion cyclotron resonance (FTICR) MS for isolation and characterization of two novel lupane triterpenoids from Paullinia pinnata L. 6P-(3 -methoxy-4 -hydroxybenzoyl)-lup-20(29)-ene-one and 6P-(3 -methoxy-4 -hydroxybenzoyl)-lup-20(29)-ene-ol, which are suspected to play a crucial role in the plant s wound healing effects [32]. The extract from Paullinia pinnata L. was subjected to an exhaustive isolation procedure starting with two consecutive column chromatography purifications on silica gel different ratios of chloroform-methanol were used as eluent. The triterpenoid-containing fraction was purified on preparative silica gel TLC plate with chloroform-methanol (9 1, v/v) as a developing solvent. The two resulting lupane bands were finally submitted to MS analyses. 

The Tibetan herb Potentilla anserina L. has been widely used in China for many thousands of years to treat hepatitis-B. Bioassay-guided fractionation of the ethanol extract of the rhizomes led to the isolation of a triterpenoid saponin (TS) that was determined to be 2a,3)3,19a-trihydroxyurs-12-en-28-oic acid )3-d-glucopyranosyl ester (Fig. 3.4 Zhao et al. 2008). Using models of HBV infection, this compound was evaluated for its effect on HBV antigene expression in the 2.2.15 cell line... 

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