Tris-EDTA buffer

Fig. 4.3.1 Effect of pH on the total light emission of phialidin (A), and the temperature stability profiles of phialidin (minute open circles) and aequorin (solid line) (B). In A, each buffer contained 0.1 M CaCl2 plus 0.1 M Tris, glycine or sodium acetate, the pH being adjusted with NaOH or HC1. In B, the photoprotein samples in 10 mM Tris-EDTA buffer solution, pH 8.0, were maintained at a test temperature for 10 min, and immediately cooled in an ice water bath. Then total luminescence activity was measured by injecting 1ml of 0.1 M CaCl2/Tris-HCl, pH 7.0, to 10 pd of the test solution. From Levine and Ward (1982), with permission from Elsevier.

Three pH values (acidic, neutral, and basic AR solution), and three heating conditions (under boiling, boiling, and pressure heating) are recommended for the basic test battery. However, alternative procedures may be applied according to laboratory facilities and routine protocols as described above. Currently, citrate buffer pH 6.0, Tris-EDTA buffer pH 8-9, and certain AR solutions at lower pH, such as boric acid pH 2-3, or acidic acid buffer pH 2, as well as 0.05% citraconic anhydride pH 7.4, may be used to evaluate the optimal AR protocol. 

Double-stranded products were purified using centrifugation dialysis following the protocol described by Allard et al.31 Purified double-stranded products were subsequently dried under vacuum and resuspended in 15 /A of IX Tris/EDTA buffer (TE). To obtain single-stranded DNA, we used 2 n 1 of the purified double-stranded PCR product as the template. The concentrations of Taq DNA polymerase, reaction buffer, dNTPs, and... 

Harvest the cells by centrifugation and resuspend the cell pellet in 1 ml of Tris/EDTA buffer (10 mM Tris, pH 7.5, 1 mM EDTA). 

The organic solvent was removed by rotary evaporation (40-45°C, 30-60 min). The plasmid DNA solution (1.5 mg/mL in Tris/EDTA buffer) was added to the dry lipid film and the lipids solubilized by vigorous agitation (see Note 3). 

Prepare the picogreen solution as described by the provider (1/200 in tris-EDTA buffer). 

Tris-EDTA buffer 50 mM Tris-HCl, pH 7.5, containing 20 mM EDTA. 

Resuspend the pellet in each tube with 9 mL Tris-EDTA buffer using a Janke Kunkel polytron tissumizer (or a similar model) equipped with a 100 mm long x 10 mm OD shaft and combine two tubes together such that each liter of original bacterial culture is now contained in four tubes. 

Carefully pour off the supernatant, resuspend the pellet in 15 mL Tris-EDTA buffer with the tissuemizer, and centrifuge again as in step 9. Repeat this three more times (see Note 34). 

FIG. 10 Typical voltammetric curves in the humidity chamber at 100% RH, 25°C. Radius of tip curvature = 10 /r,m. In all cases, the voltage was scanned from 0 V (point S) in either direction and then returned to 0 V the scan rate was 0.2 V/s. (A) Pt-Ir tip, Nation film ( 200 nm thick) on mica. (B) Pt-Ir tip, mica substrate treated with Tris-EDTA buffer solution. Curves C1-C3 first, second, and third negative scans. Curves A1-A4 first, third, fourth, and fifth positive scans after C3. Curve C4 first negative scan after A4. (C) W tip, mica substrate treated with Tris-EDTA buffer solution. (D) W tip, mica substrate treated only with water. (Reprinted with permission from Ref. 34a, Copyright 1995 American Association for the Advancement of Science.)... 

FIG. 13 Conductance values at various RH for the same mica sheet before (rectangles) and after treatment of Tris-EDTA buffer solution (asterisks) or phosphate (pluses) buffer. The conductance was measured as the slope of the current-voltage curve in the bias range of 1.5—2.0 V. 

FIG. 14 Typical parallel conductance (A) and parallel capacitance (B) vs. tip displacement curves for a blunt W tip at 100% RH and 25°C for a mica substrate treated with Tris-EDTA buffer solution. Tip bias is 3 V with respect to the Au counterelectrode. The tip approaches the substrate surface at a rate of 3 nm/s. 

FIG. 15 (A) Image of a mica surface treated with Tris-EDTA buffer solution... 

Fig. 1.11. Photoluminescence (PL) spectra from solntions containing PI and Tat-CyTAR RNA (red), Tat-CydTAR RNA (blue), SH3-CyTAR RNA (green), Aex = 380 nm, [PI] = 9.6 x 10 M in RUs. Measurements are in Tris-EDTA buffer solution (10 mM, pH = 7.4). The spectra are normalized with respect to the emission of PI [49]. Copyright Wiley-VCH Verlag GmbH Co. KGaA. Reproduced with permission...

Discard the supernatant and resuspend the mitochondrial pellet in 10 mM Tris EDTA buffer pH 8 containing 0.15M NaQ and 10 mM EDTA. The total volume should be 50 ul. 

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