Tris aminomethane salts

Ethylenediaminetetracetic acid disodium salt TRIS Tris(hydroxy)aminomethane HMTA Hexamethylenetetramine... 

Gu, L. and R. G. Strickley. 1987. Preformulation salt selection. Physical property comparisons of the tris(hydroxymethyl)aminomethane (THAM) salts of four analgesic/antiin ammatory agents with the sodium salts and the free acid iarm. Res4 255-257. 

Usually companies do not investigate other salt-forming species other than those shown in Table 3.8. However, Gu and Strickley (1987) have described the physical properties of the tris-(hydroxymethyl)aminomethane (THAM) salts of four analgesic/antiinflammatory agents with sodium salts and free acids. They concluded that the THAM salts had superior hygroscopicity properties compared to the sodium salts and did not show any loss in aqueous solubility or intrinsic dissolution rate. The one exception to this was the THAM salt of naproxen. Ketoralac is now marketed as the tromethamine or THAM salt. 

Tris Amino . [Angus] Ttis (hydroxymethyl) aminomethane pigment dispersant, neutralizing amine, corrosion inhibitor, acid-salt catalyst, pH buffer, chemical and pharmaceutic intermediate, solubili. ... 

In order to purify the RBCs, the plasma and huffy coat (white cells) are removed for further purification (see above). RBCs are then resuspended and washed three times in a physiological salt solution [PSS in mM, 4.7 KCl, 2.0 CaCh, 140.5 NaCl, 12 MgS04,21.0 tris(hydroxymethyl)aminomethane, 11.1 dextrose with 5% bovine serum albumin (final pH 7.4)]. Cells should generally be prepared and studied on the day of use within 8 h of removal from animal or human subjects. 

The n-propylammonium (Gillen and Williams, 1975), n-butylammonium (Gillen and Williams, 1975), and tris(hydroxymethyl)aminomethane (Pearson et aly 1978) salts of (+ )-l 0-camphorsulfonic acid were proposed as standards for the calibration of CD instruments. The experimental results were compared with those obtained with other standards. It was claimed that these salts can be obtained in high purity and that they are much less hygroscopic than the parent acid. 

Sodium butoxyethoxy acetate Sulfated rapeseed oil Sulfated tall oil, potassium salt Sulfated tall oil, sodium salt Talloweth Tris (hydroxymethyl) aminomethane emulsifier, cleaners oil-based PEG dioleate emulsifier, cleaning... 

For example, it has been demonstrated that normal SEC behavior can be obtained for polymethyl vinyl ether-comaleic acid using a mobile phase consisting a of pH 9 buffer system [prepared from tris(hydro-xymethyl) aminomethane and nitric acid] modified with 0.2 M LiNOj (5). Halide salts should be completely avoided they tend to corrode the stainless steel inner surfaces of the SEC system, which in turn causes injector fouling and column contamination. 

We reproduced these observations for pyridine, but we found also another buffer, trls(hydroxymethyl)aminomethane (tris), which enhanced the onset lag In both types of preparations. Interestingly, when pyridine was added on top of tris, it again failed to further prolong the onset lag in "low salt" but not in "high salt thyiakoids". This behaviour was paralleled with the ability or failure, respectively, of these buffers to decrease the extent of flash Induced pH-transients in the lumen as indicated by absorption changes of neutral red. The reported pyridine effects are therefore compatible with the delocalized coupling scheme in contrast to the Interpretation by Dllley and coworkers. 

In the pH range that fumarase exhibits activity, phosphate exists as the monobasic and dibasic salts. Since the two forms may influence the enzyme differently, pH curves were established with tris(hydroxy-methyl)aminomethane (Tris) buffer. The most striking result of these studies is that the curves obtained with malate and fumarate as substrates are quite different. The maximum rate of hydration of fumarate occurs at pH 6.5, whereas the dehydration of malate is most rapid near pH 8 (Fig. 18). 

One of the earhest extensions of the list of possible buffers for biological work was Gomori s (1946) proposal of 2,4,6-trimethylpyridine, tris(hydroxymethyl)aminomethane and 2-amino-2-methyl-l, 3-propanediol. The use of peptides such as glycylglycine and, more particularly, N-dimethyl-leucylglycine (Leonis, 1948) as biological buffers is now of little more than historical interest because a much wider range of suitable, readily water-soluble buffer substances having soluble calcium salts has been developed. 

Weak acids or weak bases in combination with their salts are, in general, adaptable for setting up buffers their greatest capacity to buffer is around their pK value. Buffers are indispensable in most biochemical work. Commonly used are buffers of phosphate, citrate, glycine and NaOH or HCl, and more recently tris(hydroxymethyi)aminomethane (or simply tris buffer ). The exact compositions of different buffer mixtures are listed in biochemical handbooks. 

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