Triamcinolone acetonide from steroid

In Figure 3, the active steroid (triamcinolone acetonide) and preservative (benzyl alcohol) are determined from a steroid cream. The higher molecular weight components of the cream base are well separated from the analytes. The ability to elute all the components of a cream or ointment in a SMGPC analysis gives an important sample preparation advantage over competing separation techniques. 

Figure 3. Determination of triamcinolone acetonide and benzyl alcohol from a steroid cream.

FIGURE 5-41. Determination of triamcinolone acetonide (peak A) and benzyl alcohol (peak B) from a steroid cream. Column 500 A Ultrastyragel 7.8 mm ID x 30 cm. Mobile phase THF. Flow rate 1 mL/min. Detection UV, 254 nm, 0.2 AUFS. (Reprinted from reference 13 with permission.)... 

For identification and differentiation from other steroids in formulations triamcinolone acetonide can be reacted with phenol and hydroquinone in a phosphoric sulfuric acid mixture producing a pink color2... 

The APC did not have a good linear relation with LD50. Dexamethasone valerate, betamethasone valerate, dexamethasone acetate and triamcinolone acetonide were approximately 1-fold more toxic and difluoro-substituted steroids were approximately 1-fold less toxic than that estimated from the partial charge. Specifically, steroids with a fluorine at the 6th position were less toxic. The correlation coefficient between LD50 and Aatomic partial charge was calculated... 

Modification of the pharmacokinetics through structural alterations has provided several new steroids with a better GR affinity and therapeutic index and a lower bioavailability than the older drugs (Fig. 33.14). The new inhaled/intranasal glucocorticosteroids like mometasone furoate, budesonide and fluticasone propionate are more lipophilic than those used in oral and systemic therapy and have greater affinity for the GR than does dexamethasone as a consequence of their greater lipophilicity (43). Several of the topical corticosteroids, such as mometasone furoate, BDP, triamcinolone acetonide, and flunisolide, were reintroduced as inhalation and intranasal dosage forms for treatment of respiratory diseases (e.g., asthma or rhinitis). Inhaled budesonide and flunisolide are readily absorbed from the airway mucosa into the blood and are rapidly biotransformed in the liver into inactive metabolites. Mometasone furoate and fluticasone propionate are very potent anti-inflammatory steroids with an oral bioavailability of less than 1%. Obviously, the risk of systemic side effects for these newer corticosteroids is greatly reduced when compared with ... 

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