Transgenics embryonic stem cells

Embryonic genome, random insertion of transgenes into, 12 454—459 Embryonic lethal, 12 460 Embryonic stem cells, gene transfer with, 12 457-459 Embryos... 

L., Mikesh, M., Marks, A., et al. (2004) Fluorescent proteins expressed in mouse transgenic lines mark subsets of glia, neurons, macrophages, and dendritic cells for vital examination. J Neurosci 24, 10999-11009. Wichterle, H., Lieberam, I., Porter, J. A. and Jessell, T. M. (2002) Directed differentiation of embryonic stem cells into motor neurons. Cell 110, 385-397. 

The second type of promoter polymorphism is a VNTR in the second intron composed of 17-bp repeats (Ogilvie et al., 1996). Ten and twelve sets of repeats are most common (Lesch et al., 1994). Studies with embryonic stem cells and transgenic embryos implicate the VNTR as playing a role in the regulation of transcription, although no definitive links are known between this VNTR and disease states (Torres et al., 2003a). 

Fig. 7.2. Steps in the production of transgenic mice by using embryonic stem cells.

Doetschman, T.C. (1991). Gene targeting in embryonic stem cells. In Transgenic Animals. N.L.First and F.P.Haseltine, eds. (New York Bntterworth-Heinemann), pp. 89 I0I. 

ES mice embryonic stem cells are an important tool foj the generation of transgenic and gene modified mice. report the effectiveness of an SdFFF cell sorter to provide from a crude ES cell preparation, a purified ES cells fraction with the highest in vivo developmental poten- tial, to prepare mice chimeras having a high percentage of chimerism. ... 

O Gorman S, Dagenais NA, Qian M, Marchuk Y. 1997. Protamine-Cre recom-binase transgenes efficiently recombine target sequences in the male germ line of mice, but not in embryonic stem cells. Proc. Natl. Acad. Sci. USA 94 14602-7... 

Pain B, Chenevier P, Samarut J. Chicken embryonic stem cells and transgenic strategies. Cells Tissues Organs, 1999 165(3/4) 212-219. 

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