Transgenic rice plants

SUDHAKAR D, FU X, SSTOGER E, WILLIAMS S, SPENCE J, BROWN D P, BHARATHI M, GATEHOUSE J A, CHRISTOU p (1998) Expression and immunolocalisation of the snowdrop Ictin, GNA in transgenic rice plants. Transgenic Res. 7 371-8... 

Shimizu M, T Kimura, T Koyama, K Suzuki, N Ogawa, K Miyashita, K Dakka, K Ohmiya (2002) Molecular breeding of transgenic rice plants expressing a bacterial chlorocatechol dioxygenase gene. Appl Environ Microbiol 68 4061-4066. 

Kawahigashi, H., Hirose, S., Inui, H., Ohkawa, H., and Ohkawa, Y., 2005, Enhanced herbicide cross-tolerance in transgenic rice plants co-expressing human CYPlAl, CYP2B6, and CYP2C19, Plant Sci. 168 773-781. 

Lucca, P., X. Ye, and I. Potykus. 2001. Effective selection and regeneration of transgenic rice plants with mannose as selective agent. Molecular Breeding 7 43—49. 

K. Shimamoto, R. Terada, T. Izawa, and H. Fujimoto. 1989. Fertile transgenic rice plants regenerated from transformed protoplasts Aatwre 338 274-276. 

Li, Z.J., Hayashimoto, A., and Murai, N. 1992. A sulfonylurea herbicide resistance gene from arabidopsis-thaliana as a new selectable marker for production of fertile transgenic rice plants. Plant Physiol., 100, 662-668. 

California-based Ventria Bioscience (Sacramento) is at the stage of field trials of transgenic rice plants expressing recombinant lactoferrin and lysozyme in rice seed (see Part IV, Chapter 8). It is worth mentioning, that these products might reach market later than planned considering difficulties with obtaining the necessary field trial permits [39-41]. 

Overexpression of Oshspl7.7 (Cl) had significantly greater resistance to heat (50°C for 2 h) and UV-B (302 nm, 3,000 mJ cm ) stresses than untransformed control plants. The level of increased thermotolerance and resistance to UV-B correlated with the level of increased Oshspl7.7 protein in the transgenic rice plants (Murakami et al. 2004). The recombinant Oshspl8.0-ll protein, a class 11 sHSP of rice, conferred both thermotolerance and UV tolerance in E. coli (Chang et al. in preparation). 

Sato, Y., and S. Yokoya. 2008. Enhanced tolerance to drought stress in transgenic rice plants overexpressing a small heat-shock protein, sHSP17.7. Plant Cell Rep. 27 329-334. 

FIG U RE 9.4 The bacterial leaf blight symptom caused by Xanthomonas oryzae pv. oryzae on wild-type (A) andp p-transgenic rice plants (B). 

GM-CSF produced in the seeds of transgenic rice plants. Transgenic. Res., 16, 713-721. 

Wu, C.Y. Suzuki, A. Washida, H. Takaiwa, F. (1998). The GCN4 motif in a rice glutehn gene is essential for endosperm-specific gene expression and is activated by Opaque-2 in transgenic rice plant. Plant Journal, Vol.l4, No.6, 0une 1998), pp. 673-... 

Kawahigashi H, Hirose S, Ohkawa H, Ohkawa Y (2003) Transgenic rice plants expressing human CYPlAl exude herbicide metabolites from their roots. Plant Sci 165 373-381... 

A, a transgenic rice plant (To) transformed with the mutant ALS gene... 

Transgenic rice plants at 5- to 6-leaf stage were treated with bispyribac-oodium (1 kg a.i /ha). The photograph was taken two months after bispyribac-sodium treatment Plant length of the transgenic rice piant marked by A was 88 cm. 

Evaluation of Herbicide Metabolism in Transgenic Rice Plants Expressing CYPlAl and CYP2B6... 

We have produced transgenic rice plants eiqnressing CYPIAI and CYP2B6 these transgenic rice plants showed remarkable cross-tolerance toward herbicides and enhanced herbicide detoxification, a property that should be useful in phytoremediation. 

Production of Transgenic Rice Plants Expressing Human P450 Genes... 

Figure I. T-DNA region of pIESIAI andpIJ2B6plasmids used to express human CYPl A1 and CYP2B6 in transgenic rice plants. RB, right border LB, left border NOS, nopaline synthase promoter NT, nopaline synthase terminator NPTII, neomycin phosphotransferase II 35S, cauliflower mosaic virus (CaMV) 35S promoter E7, seven-enhancer region (-290 to -90) from CaMV 35S promoter AMV-5 VTR, alfalfa mosaic virus 5 -untranslated region HPT, hygromycin B phosphotransferase.

Figure 2, Phytotoxicity of various herbicides toward CYPlAl and CYP2B6 transgenic rice plants in germination tests in MS medium, C, Nipponbare without herbicide (control) N, Nipponbare with herbicide lA, CYPlAl plants with herbicide 2B, CYP2B6 plants with herbicide.

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