Transformed hairy root

Index Entries Panax ginseng transformed hairy root inoculum condition inoculum age root culture. 

Plants are known as a potential source of a large number of important biochemical constituents (1-3). In recent years, transformed hairy roots have been studied as a potential large-scale source for production of plant-derived useful compounds such as pharmaceuticals. They have several advantages compared to plant cell suspension culture, such as high growth rate, high and stable secondary metabolite productivity, autotrophy of... 

Differentiation into root-type tissues is apparently necessary for alkaloid production. Consequently the transformation of solanaceous plants with Agrobacterium rhizogenes, yielding so-called hairy roots which can easily be grown in vitro, was a logical step in efforts to produce tropane alkaloids in vitro 135,268,269,293,296,314-316,319,328,329, 338,347,348). In Table XIX results obtained with Agrobacterium rhi-zogenej-transformed hairy root cultures are summarized. 

Cell mass production and metabolite production by cells developed over the last 50 years. Muir et al. reported the first suspension culture of plant cells and the first cultivation of cells in bioreactors in 1955 by Melchers and Engelmann. The development of bioreactors since this time was extremely fast, from small flask type to multicubic meters. One of the largest bioreactors in the production of alkaloids by transformed hairy roots is reported to be of 500 m in size and has been used in a pilot scale in 1990 by the research group of Wilson. In recent studies, the different bioreactors have been compared to produce galanthamine from shoots of... 

Zarate R (1999) Tropane alkaloid production by Agrobacterium rhizogenes transformed hairy root cultures of Atropa baetica Willk (Solanaceae). Plant Cell Rep 18(5) 418 23. doi 10.1007/S002990050596... 

Sheki H, Nishizawa T, Tanaka N, Niwa Y, Yoshida S, Mmanaka T (2005) Hairy root-activation tagging a high-throughput system for activation tagging in transformed hairy roots. Plant Mol Biol 59 793-807... 

Kondo, T, Inoue, M., Mizukami, H., Ogihara, Y Cytotoxic activity of hryonohc add isolated from transformed hairy roots of Trichosanthes kirilowii var. japonica. Biol. Pharm. Bull. 18(9), 726-729 (1995)... 

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