Total internal reflectance fluorescence reactions

Tokunaga M, Kitamura K, Saito K, Iwane A H and Yanagida T 1997 Single molecule imaging of fluorophores and enzymatic reactions achieved by objective-type total internal reflection fluorescence microscopy Biochem. Biophys. Res. Commun. 235 47-53... 

The diffusion-related molecular processes occurring within a Cig stationary-phase have also been investigated using pyrene as a fluorescent probe [169]. Particular spectral bands were attributed to pyrene excimers formed in a diffusion-limited reaction. Rate constants for this formation were then used to estimate the microviscosity of the stationary-phase. A similar application of total internal reflection fluorescence... 

Picosecond time-resolved total internal reflection fluorescence spectroscopy was applied to analyze the proton-transfer reaction of INpOH in water-sapphire interface layers [206], The rate constant of the proton-transfer reaction from excited neutral species became slow in the interface layer as compared with that in the bulk aqueous solution and decreased smoothly with increasing penetration depth in the interfacial layer up to 100 nm. The anomaly was interpreted in terms of rotational fluctuations of water aggregates in the interface layer. 

Total Internal Reflection Fluorescence Microscopy (TIRFM) is another useful tool for studying the reactions of individual molecules adsorbed, adhered or bound to surfaces. Typical applications are membrane fusion of vesicles [177], conformational and orientation changes [178] and lateral mobility of molecules [179],... 

SHG) [610], total internal reflection fluorescence (TIRF) spectroscopy [611], UV/Vis spectroscopy, IR spectroscopy, ellipsometry [612], quartz crystal microbalance (QCM) [613], STM [614], and AFM (see Refs. [618-623] for review of in sitn spectroscopies). From those, only the vibrational methods (SHG, SERS, and IR spectroscopy) are able to provide information on both the chemical composition and the strnctnre of the species adsorbed. The IR SEC experiment is simpler and more accessible than SHG and SERS, making IR spectroscopy the dominant tool for stndying electrochemical reactions at metallic electrodes [616, 617, 624-641]. 

Tokunaga, M., et al.. Single molecule imaging of fluorophores and en2ymatic reactions achieved by objective-t5fpe total internal reflection fluorescence microscopy. Biochemical and Biophysical Research Communications, 1997. 235(1) p. 47-53. 

V. Hlady, J. N. Lin, and J. D. Andrade, Spatially resolved detection of antibody-antigen reaction on solid/liquid interlace using total internal reflection excited antigen fluorescence and charge-coupled device detection, Biosens. Bioelectron. 5,291-301 (1990). 

T. E. Starr and N. L. Thompson, Total internal reflection with fluorescence correlation spectroscopy Combined surface reaction and solution diffusion, Biophys. J. 80, 1575-1584 (2001). 

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