Tomato transgenic

Figure 4. Inhibition of A. nigerand F. moniliforme endopolygalacturonase by PGIP purified fom tomato transgenic plants (A) and from bean (B).

Fig. 51.8 Comparison of coumaric acid and ftmilic acid accumulation in wild-lype 35SS and LoxS tomato transgenic open flowers...

BIRD C R, RAY J A, FLETCHER J D, BONIWELL J M, BIRD A S, TEULIERES C, BLAIN I, BRAMLEY P M and SCHUCH w (1991) Using antisense RNA to study gene function inhibition of carotenoid biosynthesis in transgenic tomatoes , BioTechnology, 9, 635-9. 

FRASER P D, ROMER S, SHIPTON C A, MILLS P B, KIANO I W, MISAWA N, DRAKE R G, SCHUCH W and BRAMLEY p M (2002) Evaluation of transgenic tomato plants expressing an additional phytoene synthase in a fruit-specific manner , Proc Natl Acad Sci, 99, 1092-7. 

FRAY R, WALLACE A, FRASER P D, VALERO D, HEDDEN P, BRAMLEY P M and GRIERSON D (1995) Constitutive expression of a fruit phytoene synthase gene in transgenic tomatoes causes dwarfism by redirecting metabolites from the gibberellin pathway . Plant J, 8, 696-701. 

ROMER S, FRASER P D, KIANO J, SHIPTON C A, MISAWA N, SCHUCH W and BRAMLEY P M (2000) Elevation of the provitamin A content of transgenic tomato plants . Nature Biotech, 18, 666-9. 

Plant phytoene synthase (Psy) has been used in a variety of transgenics. As noted above, P yl over-expression under a strong constitutive promoter caused a decrease in carotenoid accumulation, probably due to transcription silencing. Similarly, over-expression of the gene sequence backward (antisense) also silenced activity. In another approach to over-expression of tomato 1 in fruits, a synthetic alternative in which the third position of each codon was changed in order to avoid transcriptional silencing was successful in conditioning an increase in carotenoid accumulation. 

The availability of precursor IPP may ultimately be most influential over accumulation of carotenoid metabolites. While over-expression of DXS and DXR in color complementation systems leads to hyperaccumulation of carotenoids (discussed in Section 5.3.3.3), over-expression of plant Dxs genes has not always been effective. Over-expression of DXS resulted in increased carotenoid accumulation in transgenic tomato and Arabidopsis, but over-expression of daffodil DXS in rice endosperm did not increase pigment accumulation. ... 

Bramley, P. et al.. Biochemical characterization of transgenic tomato plants in which carotenoid synthesis has been inhibited through the expression of antisense RNA to pTOM5, Plant J. 2 (3), 343, 1992. 

Long, M. et al.. Metabolite profiling of carotenoid and phenolic pathways in mntant and transgenic lines of tomato identification of a high antioxidant fmit line. Phytochemistry, 67, 1750, 2006. 

Expression of polygalacturonase and pectinesterase in normal and transgenic tomatoes. 

Figure 2 Pectinesterase activity in transgenic tomato finit. Activity is expressed as...

Taylor,J.E., Tucker,G.A., Lasslett,Y., Smith,C.J.S., Amold,C.M., Watson,C.F., Schuch,W., Grierson,D and Roberts,J.A (1990) Polygalacturonase expression during leaf abscission of normal and transgenic tomato plants. Planta. 183.133-138. 

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