Tobacco isolated

Schmeltz, L, A. Wenger, D. Hoffmann, and T.C. Tso Use of radioactive tobacco isolates for studying the formation of selected smoke components 30th Tobacco Chemists Research Conference, Program Booklet and Abstracts, Vol. 30, Paper No. 35, 1976, p. 26 Chemical studies on tobacco smoke. 53. Use of radioactive tobacco isolates for studying the formation of smoke components J. Agr. Food Chem. 26 (1978) 234-239. 

Schumacher, J.N. Turkish tobacco. Isolation, characteriza-hon, and synthesis of 6-acetyl-2,3,4-tr -rf-3-methylvaleryl-P-D-glucopyranoside RDR, 1960,No. 16,May 12,seewww. ijrtdocs.com 500934742 -4763 The isolahon of 6-O-acetyl-2,3,4-0-tm-[(-l-)-3-methylvaleryl]-P-ZJ-glucopyranoselrom tobacco Carbohydrate Res. 13 (1970) 1-8. 

Isolates from Indian tobacco Q obelia inflata L.), as a cmde mixture of bases, have been recognized as expectorants. The same (or similar) fractions were also used both in the treatment of asthma and as emetics. The principal alkaloid in T. inflata is lobeline (49), an optically active tertiary amine which, unusual among alkaloids, is reported to readily undergo mutarotation, a process normally associated with sugars. Interestingly, it appears that the aryl-bearing side chains in (49) are derived from phenylalanine (25, R = H) (40). 

Comparison of these results indicates identity of the two substances isolated independently by Orekhov and Ehrenstein, but Spiith and Kesztler have suggested that Pictet s nicoteine and Ehrenstein s base, consisted of impure Z-anatabine (p. 46). In this connection it may be noted that Ehrenstein s base was laevorotatory in acid solution, whereas Salts of anabasine are dextrorotatory. These authors have themselves isolated Z-anabasine from tobacco. The identity of synthetic 2-(3 -Pyridyl)piperidine with dZ-anabasine seems to have been definitely established. Anabasis aphylla is the source of the anabasine raanufac-... 

Nicotelline, CjoHgNj. This base, isolated by Pictet and Rotschy, forms colourless needles, m.p. 147-8°, b.p. above 300° its aqueous solution is neutral to litmus. Unlike other tobacco bases it yields a sparingly soluble, crystalline dichromate. It does not decolorise acid permanganate, and appears not to be a pyrrole derivative. ... 

Alkaloids of Tobacco Smoke. The following alkaloids, apart from normal alkaloidal constituents of tobacco, have been isolated by Wenusch and Schoeller from tobaceo smoke. 

Efforts are still being made to estimate that elusive notion quality in smoking tobacco by chemical analysis it does at least seem to be clearly established that a low content of protein and of nicotine is desirable, and in that connection the isolation by Bucherer of several species of... 

B Nicotine is a diamino compound isolated from dried tobacco leaves. Nicotine has two rings and M + = 162.1157 by high-resolution mass spectrometry. Give a molecular formula for nicotine, and calculate the number of double bonds. 

In addition to the proteins mentioned above, there have been many reports of the induction of pathogenesis-related proteins (PRPs) in a number of plant species infected by viruses, viroids, bacteria or fungi (Van Loon, 1985). It has been shown that in cultured parsley cells, increased transcription of two PRP genes occurs within a few minutes of exposure to fungal elicitors (Somssich et al., 1986). Although several PRPs from tobacco have been purified and their cDNAs isolated (Hooft van Huijsduij-nen. Van Loon Bol, 1986), the exact action of these proteins remains unclear. 

Chopra NM, Campbell BS, Hurley JC. 1978. Systematic studies on the breakdown of endosulfan in tobacco smokes Isolation and identification of the degradation products from the pyrolysis of endosulfan I in a nitrogen atmosphere. J Agric Food Chem 26 255-258. 

The first step in the analysis is extraction of the tobacco with buffer solution (pH 4.5) containing 20 mM ascorbic acid. The nitrosamines are then concentrated by partition with dichloromethane, and a chromatographic clean-up on alumina. In the final step, the concentrate is analyzed by GC-TEA and confirmation of the nitrosamines is obtained by GC-MS (O. If isolated amounts of the nitrosamines are below levels needed for GC-MS confirmation, we employ confirmatory techniques proposed by Krull et a. ( 5). 

Poindexter, E., Carpenter, R. The isolation of harmane and norharmane from tobacco and cigarette smoke. Phytochemistry. 1 215, 1962. 

The purple speck disease of soybeans is caused by the fungus Cercospora kikuchii, and additional cash crops such as tobacco, com, sugar, and coffee are damaged by fungi from the genus Cercospora [3]. Cercosporin (3, Chart 7.1), initially isolated in 1957 from Cercospora kikuchii and subsequently from other Cercospora species, was found to be the phototoxin responsible for the destructive nature of the pathogen [4]. For these reasons, the natural product was extensively studied, yet its structure was not elucidated until the 1970s [5]. 

Most protocols for the isolation of protoplasts from Cowpea, Petunia, or Tobacco mesophyll cells are based on [93, 94] and others [95-97], After the isolation of protoplasts from the cell type of choice, the transfection is carried out as follows (based on [51, 96]) ... 

Human papilloma virus type 11 major capsid protein LI Tobacco leaf, potato tuber Reacted well with assembled capsids and isolated LI capsomers. Immunogenic in mice when administered parenterally with subsequent oral boosting. 99... 

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