Time-resolved fluorescence spectroscopy applications

It is confirmed that the polymer matrix around ablated area was also affected strongly by laser ablation. The change of the matrix properties are brought about over a few tens of pin. This type of information is basically important and indispensable for practical applications such as excimer laser lithography. The time-resolved fluorescence spectroscopy is one of the powerful characterization methods for ablated polymer matrix. 

Lakowicz J. R. and Szymacinski H. (1996) Imaging Applications of Time-Resolved Fluorescence Spectroscopy, in Wang X. F. and Herman B. (Eds), Fluorescence Imaging Spectroscopy and Microscopy, Chemical Analysis Series, Vol. 137, John Wiley ... 

Emerging Biomedical Applications of Time-Resolved Fluorescence Spectroscopy... 

E. P. Diamindis, Immunoassays with time-resolved fluorescence spectroscopy Principles and applications, Clin. Biochem. 21, 139-150(1988). 

Volume 4 is intended to summarize the principles required for these biomedical applications of time-resolved fluorescence spectroscopy. For this reason, many of the chapters describe the development of red/NIR probes and the mechanisms by which analytes interact with the probes and produce spectral changes. Other chapters describe the unique opportunities of red/NIR fluorescence and the types of instruments suitable for such measurements. Also included is a description of the principles of chemical sensing based on lifetimes, and an overview of the ever-important topic of immunoassays. 

In addition to fluorescence intensity and polarization, fluorescence spectroscopy also includes measurement of the lifetime of the excited state. Recent improvements in the design of fluorescence instrumentation for measuring fluorescence lifetime have permitted additional applications of fluorescence techniques to immunoassays. Fluorescence lifetime measurement can be performed by either phase-resolved or time-resolved fluorescence spectroscopy. 

The application of vb-DMASP to MIPs was continued in subsequent works [64, 65], In these investigations, time-resolved fluorescence spectroscopy was applied to study bulk fluorescent MIP. The imprinted polymer fluorescence quenching with increasing concentrations of aqueous cAMP was determined from the fluorescence lifetime parameters. Two components in the fluorescence decays were identified and assigned to two different types of cavities present in the polymer matrix. One was accessible and open to binding, whereas the other was inaccessible, being buried inside the bulk polymer. The fluorescence lifetime decreased due to the increase in the concentration of the initial target analyte. However, the accessible... 

Basic principles and applications of time-resolved fluorescence spectroscopy have been outlined in a very illustrative way by Valeur [16]. Although punctiform spectroscopy is still the best way to get a detailed knowledge of all the important parameters that characterize fluorescence emission (exact spectral properties, decay time behavior, polarization), imaging is always preferred whenever the localization of the distribution of any biomolecule of interest is required or a great number of samples have to be analyzed [22]. 

Diaraandis E. Immunoassays with time resolved fluorescence spectroscopy Principles and applications. 

Time-resolved luminescence spectroscopy complements the steady-state method and can provide essential kinetic information about the decay of excited states. Application of time-resolved fluorescence spectroscopy for analytical chemistry, where low concentrations might require the use of long... 

The application of time-resolved fluorescence spectroscopy to studies of excimer formation and energy transfer in PACE and P2NMA provides an overview of the emitting species present and the dynamics of energy relaxation in these polymers. The results of fluorescence decay analyses suggest that kinetic models which have been proposed to explain monomer/excimer kinetics may require further refinanent. 

Since NSOM utilizes optically based contrast it has the potential to exploit optical spectroscopies with resolution comparable to the probe dimensions, i.e. tens of nanometres. For example, steady state and time-resolved fluorescence spectroscopy and Raman spectroscopy have been demonstrated with NSOM. Despite some notable successes it is important to note that the combination of the difficulty in the interpretation of NSOM data and the often poor optical efficiency of NSOM probes, presently makes the application of NSOM the most challenging form of SPM. 

Neumann M., Herten D.-P. and Sauer M. (2001) New Techniques for DNA Sequencing Based on Diode Laser Excitation and Time-Resolved Fluorescence Detection, in Valeur B. and Brochon J. C. (Eds), New Trends in Fluorescence Spectroscopy. Applications to Chemical and Life Sciences, Springer-Verlag, Berlin, pp. 303-29. 

The technique of transient grating spectroscopy has been reviewed, with particular emphasis on its application to monitoring non-radiative deactivation. A unified theory of time-resolved fluorescence anisotropy and Stokes shift spectroscopy has appeared. A separate review has considered the chemical and photophysical events occurring from upper excited states as accessed by multiphoton absorption techniques. ... 

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