Fluorescence time-resolved detection

Brand L, Eggeling C, Zander C, Drexhage K FI and Seidel CAM 1997 Single-molecule identification of coumarin-120 by time-resolved fluorescence detection comparison of one- and two-photon excitation in solution J. Chem. Phys. A 101 4313-21... 

Mosconi, D., Stoppa, D., Pancheri, L., Gonzo, L. and Simoni, A. (2006). CMOS single-photon avalanche diode array for time-resolved fluorescence detection. IEEE ESSCIRC. 564-67. 

Neumann M., Herten D.-P. and Sauer M. (2001) New Techniques for DNA Sequencing Based on Diode Laser Excitation and Time-Resolved Fluorescence Detection, in Valeur B. and Brochon J. C. (Eds), New Trends in Fluorescence Spectroscopy. Applications to Chemical and Life Sciences, Springer-Verlag, Berlin, pp. 303-29. 

The approach described above is the simplest variant of time resolved fluorescence detection for the measurement of collision processes. If we choose to excite state A and detect the fluorescence from a second state B, the fluorescence intensity has the form... 

Fig. 16.9 Recording of fine structure beats in the Na 9d level (averaging of 1000 runs) obtained by time resolved fluorescence detection. Trace (a) signal obtained with configuration of polarizers ej 1 ed as shown in the upper inset trace (b) signal obtained with...

Finally, the use of CCD cameras as detectors in combination with short-pulsed light sources enables not only spatially resolved imaging, but also a time-resolved fluorescence detection. Time resolution offers two different new alternatives ... 

HTRF is a homogeneous technology which combines an FRET process with time-resolved fluorescence detection to probe biomolecular interactions. This combination is made possible through the use of a long lifetime fluorescent FRET donor, a lanthanide cryptate. Cryptates are formed by the inclusion of a rare earth ion e.g. 

Moreover, the HTRF signal readout minimizes the instances of compound interference in HTS the ratiometric signal measurement overcomes the influence of highly coloured compounds while the time-resolved fluorescence detection discards... 

Many studies used radiation chemistry to produce the radical and radical cations and anions of various dienes in order to measure their properties. Extensive work was devoted to the radical cation of norbomadiene in order to solve the question whether it is identical with the cation radical of quadricyclane . Desrosiers and Trifunac produced radical cations of 1,4-cyclohexadiene by pulse radiolysis in several solvents and measured by time-resolved fluorescence-detected magnetic resonance the ESR spectra of the cation radical. The cation radical of 1,4-cyclohexadiene was produced by charge transfer from saturated hydrocarbon cations formed by radiolysis of the solvent. In a similar system, the radical cations of 1,3- and 1,4-cyclohexadiene were studied in a zeolite matrix and their isomerization reactions were studied. Dienyl radicals similar to many other kinds of radicals were formed by radiolysis inside an admantane matrix. Korth and coworkers used this method to create cyclooctatrienyl radicals by radiolysis of bicyclo[5.1.0]octa-2,5-diene in admantane-Di6 matrix, or of bromocyclooctatriene in the same matrix. Williams and coworkers irradiated 1,5-hexadiene in CFCI3 matrix to obtain the radical cation which was found to undergo cyclization to the cyclohexene radical cation through the intermediate cyclohexane-1,4-diyl radical cation. 

Hakala, H., and Lonnberg, H. (1997) Time resolved fluorescence detection of oligonucleotide hybridization on a single microparticle covalent immobilization of oligonucleotides and quantitation of a model system. Bioconjugate Chemistry, 8, 232 237. 

N. Neumann, D-P. Herten, M. Sauer, New techniques for DNA sequencing based an diode laser excitation and time-resolved fluorescence detection, in B.Valeur, J.C. Brochon (eds.), New trends in fluorescence spectroscopy. Springer Verlag Berlin, Heidelberg, New York, 303-329 (2001)... 

M. Prummer, C. Hiibner, B. Sick, B. Hecht, A. Renn, U.P. Wild, Singlemolecule identification by spectrally and time-resolved fluorescence detection, Anal. Chem. 72, 433-447 (2000)... 

Miller, K. J. and Lytle, F. E., Capillary zone electrophoresis with time-resolved fluorescence detection using a diode-pumped solid-state laser, J. Chromatogr., 648, 245, 1993. 

Schmittel and Burghart have produced an excellent review, Understanding Reactivity Patterns of Radical Cations . The detection and observation of radical cations are highly desirable, but also have often been difficult, owing to their short lifetimes. West and Trifunac have developed a technique known as time-resolved fluorescence-detected magnetic resonance (FDMR) that gives the maximum possible time resolution for EPR and thus a window into fast radical cation reactions in solution. They have also linked this work with matrix isolation of radical cations using microporous zeolite reactors. 

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