Time-resolved detection

Miller R J and Closs G L 1981 Application of Fourier transform-NMR spectroscopy to submicrosecond time-resolved detection in laser flash photolysis experiments Rev. Sc/. Instrum. 52 1876-85... 

The unloading wave itself provides a direct measure of the strength at pressure from the shape of the release wave. Such a measurement requires time-resolved detection of the wave profile, which has not been the usual practice for most strong shock experiments. 

If Time resolved detection at 96 spots S Time resolution 25 s > Regeneration results in original values for optical thickness... 

Pulse radiolysis, using as time-resolved detection methods optical absorption, luminescence, electrical conductivity or electron spin resonance can be expected to give information on the formation of transient or permanent radiation products and on their movement. 

Morrison LE (1988) Time-resolved detection of energy transfer theory and application to immunoassays. Anal Biochem 174 101-20... 

Embedding of pinacyanol in a three-dimensional polyphenylene dendrimer results in a red-shift of absorption (from 600 to 620 nm) and emission (from 625 to 648 nm) maxima and in an increase of the quantum yield from 9 x KT4 for free dye in water to 1.4 x 10 2 after insertion in the dendrimeric architecture [71]. This dendrimer was used to develop two FRET systems utilizing cyanine dyes as the donor (DTCI) and the acceptor (pinacyanol and 26a) molecules [72], The FRET system allows the time-resolved detection, where energy transfer can be observed at the single-molecule level. 

The time-resolved detection of fluorescence microscopy has been achieved conventionally by these two methods. Therefore, the time resolution of the fluorescence microscope was limited to several tens of picoseconds or nanoseconds (Krishnan et al. 2003 McLoskey et al. 1996). 

Becker, W., Hickl, H., Zander, C., et al. 1999. Time-resolved detection and identification of single analyte molecules in microcapillaries by time-correlated single-photon counting (Tcspc). Rev. Sci. Instrum. 70 1835. 

Another possible solution that has been under development for three decades is to use a pulsed laser and time-resolved detection to allow the Raman photons to be discriminated from the broad luminescence background. The Raman interaction time is virtually instantaneous (less than 1 picosecond), whereas luminescence emission is statistically relatively slow, with minimum hundreds of picoseconds elapsing between electronic excitation and radiative decay. If we illuminate a sample with a very short (= 1 ps) laser pulse, all of the Raman... 

The Experimental Technique chapter describes our experimental setup with the following main parts laser source (Ar, excimer, Nd-YAG, nitrogen, dye, OPO), imaging monochromator, gated detector (Intensified Charge Coupled Device) and computer with corresponding software. The main features of the experimental devices are described, which enable us to accomphsh time-resolved detection. 

Gruber, H. J. Kada, G. Pragl, B. Riener, C. Hahn, C. D. Harms, G. S. Ahrer, W. Dax, T. G. Hohenthanner, K. Knaus, H. G. Preparation of thiol-reactive Cy5 derivatives from commercial Cy5 succinimidyl ester. Bioconjug. Chem. 11(2), 161-166. Gudgin Dickinson, E. F. Poliak, A. Diamandis, E. P. Time-resolved detection of lanthanide luminescence for ultrasensitive bioanalytical assays. J. Photochem. Photobiol. B Biol. 1995,27, 3-19. 

The most commonly used method is the direct measurement of a decay rate by pulsed excitation and time resolved detection. The most straightforward example of this technique is laser induced fluorescence applied to alkali Rydberg atoms. Alkali atoms are typically contained in a glass cell, which also holds a known pressure of perturber gas. The alkali atoms are excited to the Rydberg state at time t = 0 and the time resolved fluorescence from the Rydberg atoms is detected... 

Direct time-resolved detection of singlet oxygen in zeolite-based photocatalysts. Langmuir, 24 (9), 4478-4481. 

For example, [94] reports a direct process for investigating ZnO polarity based on the analysis of time-of-flight spectra in ion-scattering experiments low-energy ion beams are scattered over the surface of the layer, causing it to be sputtered by the ion bombardment. The detector allows the time-resolved detection of the Zn-particle stream and of the scattered primary ions. The extent of O termination and Zn termination for the layer can be calculated from the difference between these signals. 

Garland JE, Pettit CM, Roy D (2004) Analysis of experimental constraints and variables for time resolved detection of Fourier transform electrochemical impedance... 

Time-Resolved Detection of Intermolecular Interaction of Photosensor Proteins... 

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