Time-lapse imaging

Fig. 8. Time lapse images of two MMTV arrays visualized with GFP-GR. Time in minutes after addition of 100 nM dexamethasone is indicated. Some arrays become very extended (3-10 pm, B), whereas some cells exhibit a less extended array (< 3 pm, A) (from Ref [68]). Scale bar 1 pm.

Tirlapnr, U. K., and Kbnig, K. 2001. Femtosecond near-infrared lasers as a novel tool for non-invasive real-time high-resolntion time-lapse imaging of chloroplast division in living bnndle sheath cells of Arabidopsis. Planta 214 1-10. 

Keratin IFs exhibit different motile behaviors compared with type III and IV IFs, and continue to move in the absence of MT in cells treated with MT inhibitors, such as nocodazole. It has been proposed that actin may be involved in their transport (Yoon et al., 2001). Time lapse imaging of keratin-GFP has revealed that keratin IF formation begins in the cell cortex in a region enriched in actin and required for both intact MT and actin, and that actin governs the organization and movement of keratin in Xenopus egg extracts (Weber and Bement, 2002). Less is known about specific mediators of these interactions, although the fact that myosin Va associates with NFs raises the interesting possibility that other such interactions may exist for keratins. 

Fig 15 An image (7.3 nm x 6.9 nm) of sulphur self-organized in hexagons and equilateral triangles made of 18 sulphur adatoms. At room temperature and fixed S/Cu stoichiometry (0cu 0.03 ML for this image) the observed structural patterns fluctuate for hours. Lower two time-lapse images (3.5 nm x 3.3 nm) taken 50 s apart show formation of new equilateral triangles. Reprinted from ref. [23]. 

Figure 9. Time lapse images a cube being released from the docked position (a) initially, there is now flow in/out of the docking pedestal at t = 0 s flow out of the pedestal is initiated (b) the flow rejects the block (c) the blocks moves out into the bulk flow.

Zebrafish have emerged as a powerfiil model organism to study neutrophil chemotaxis and inflammation in vivo. Studies of neutrophil chemotaxis in animal models have previously been hampered both by the limited number of specimens available for analysis and by the need for invasive procedures to perform intravital microscopy. Due to the transparency and cell permeability of zebrafish embryos these limitations are circumvented, and the zebrafish system is amenable to both live time-lapse imaging of neutrophil chemotaxis and for screening of the effects of chemical compounds on the inflammatory response in vivo. Here, we describe methods to analyze neutrophil-directed migration toward wounds using both fixed embryos by myeloperoxidase activity assay, and live embryos by time-lapse microscopy. Further, methods are described for the evaluation of the effects of chemical compounds on neutrophil motility and the innate immune responses in zebrafish embryos. 

Set a sequential time lapse image acquisition (a single time point is illustrated in Fig. 2). 

Simultaneous imaging of two different molecules provides an opportunity to more precisely determine the order of translocation and activation events (33). Simultaneous time-lapse imaging has been used in several biology fields, such as for the visualization of ions and pH, and this approach is gaining increasing attention and... 

Analyzing Ras and Rapl Activation by Time-Lapse Imaging... 

These techniques require a confocal microscope equipped with a YFP filter and the capability to perform time-lapse imaging in conjugation with photobleaching. In addition, a high numerical... 

FRET Time-Lapse Imaging in Live Cells... 

FRET imaging can monitor dynamic interactions between a FRET donor (ECFP) and an acceptor (EYFP), which are fused to the protein of interest, in live cell experiments. As an example, we show the FRET changes of a fluorescent biosensor for Ca +, came-leon, using the spectral detector of the Zeiss LSM 510 META in a time-lapse imaging experiment. Cameleon is a chimeric protein that consists of ECFP, the calmodulin-binding peptide M13... 

FRET time-lapse imaging method to measure the induced Ca + response by monitoring intensity changes of ECFP and EYFP in single live HEK293 cells after IL-8 (chemokine) activation of the CXCRl receptor (Fig. 2). 

The time-lapse imaging data are quantitated using MetaMorph software (Figs. 7 and 8). 

Barretto, R.P., et al. Time-lapse imaging of disease progression in deep brain areas using fluorescence microendoscopy. Nature Medicine 17(2), 223-228 (2011)... 

Fig. 4 (a) Possible droplet positions and directions in three-electrode mixing, (b) Time-lapse images of three-electrode mixing at 8 Hz... 

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