Tight junction proteins claudins

Guan, X. Inai, T. et al. (2005). "Segment-specific expression of tight junction proteins, claudin-2 and -10, in the rat epididymal epitheliiun." Arch Histol Cytol, 68(3), 213-25. 

Fig. 3 (continued) assayed by TUNEL. Numerous TUNEL-positive cells were observed after treatment with gpl 20, while almost no apoptotic cells were seen in cultures that were not incubated with gpl 20 (p<0.001 serum-free media (control) vs. 200 ng/ml gpl 20 in serum-free media for 24 h). Almost all occludin-positive cells were TUNEL positive for 200 ng/ml gpl 20. Control with buffer only and no enzyme showed no TUNEL staining (not shown), (b) After intra-caudate-putamen (CP) injection of HIV-1 500 ng gpl 20, fewer occludin-positive structures were seen, (c) Decrease in the number of claudin-5 (a tight junction protein)-positive structures parallels the leakage of Evans blue (EB) in CP previously injected with HIV-1 gpl 20. (d) Reduction in the number of laminin (a basement membrane proteinj-positive structures observed after intra-CP injection of HIV-1 gpl 20, suggesting a degradation of the basement membrane protein (modified from [105])... 

Alveolar epithelial cells (AECs) are connected with each other by various epithelial cell-cell contacts (i.e., tight junctions, adherens junctions and others). These contacts comprise several groups of proteins, such as occludin, zonula occludens (ZO-1, -2, -3), claudins, tricellulin, E-cadherin and intercellular adhesion molecule-1 (ICAM-1) [13-15], Their regulation and interplay, which has influence on epithelial barrier properties, however, is largely unknown to date. 

Furuse M, Fujita K, Hiiragi T, Fujimoto K, and Tsukita S [1998] Claudin-1 and -2 Novel integral membrane proteins localizing at tight junctions with no sequence similarity to occludin. J Cell Biol 141 1539-1550... 

Morita K, Furuse M, Fujimoto K, and Tsukita S [1999] Claudin multigene family encoding four-transmembrane domain protein components of tight junction strands. Proc Natl Acad Sci USA 96 511-516... 

Kubota K, Furuse M, Sasaki H, Sonoda N, Fujita K, Nagafuchi A, and Tsukita S [1999] Ca2" " -independent cell-adhesion activity of claudins, a family of integral membrane proteins localized at tight junctions. Curr Biol 9 1035-1038... 

Tight junctions form selective barriers that regulate paracellular transport across epithelia and endothelia. Tight junctions are composed of transmembrane proteins (occludin, claudins, and JAMs) linked to the actin cytoskeleton through cytoplasmic ZO proteins, whereas adherence junctions are composed of the nectin-afadin system and the E-cadhcrin catenin system [143]. These cell cell adhesion proteins create the barrier and regulate electrical resistance, size, and ionic charge selectivity [144], Several experimental procedures have... 

Mitic LL, van Itallie CM (2001) Occludin and claudins transmembrane proteins of the tight junction. In Cereijido M, Anderson JM (eds) Tight Junctions (2nd edn.). CRC, Boca Raton, pp 213-230... 

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