Three-Dimensional 3D Culture of hPSCs on Biomaterials

Several 3D culture strategies for hPSCs have been established (a) cell culture in macroporous scaffolds, (b) cell culture on nanofibers or microfibers, (c) self-aggregated spheroid (cell aggregate) culture, (d) cell culture on microcarriers, and (e) microencapsulated cell culture in suspended hydrogels [97,98]. 

Frequently, hPSCs have been cultmed on microcarriers in suspension because a high surface-to-volume ratio can be achieved for a microcarrier cell culture system. Microcarriers have been used for the scalable cultme of anchorage-dependent cells. 

Cell Line Microcarrier Name Materials of Microcarrier Shape Coating Materials on Microcarriers Medium Fold Expansion (Doubling Time ) Longest Culture Time Ref. (Year) 

Several other researchers reported hESCs to be cultured on microcarriers without a Matrigel or ECM coating [103,107,108]. However, the hESC culture period of those studies was reported to be a relatively short period (no more than two to six passages). Therefore, most researchers [65,105,113] currently use a Matrigel or ECM coating on the microcarriers to expand hPSCs and to maintain their pluripotency. 

Cytodex 1 Cross-linked dextran with N, N-diethylaminoethyl groups Fair Poor 

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