Thin-layer detecting insecticides

Ackermann H. 1966. [Enzymatic detection of organophosphorus insecticides using thin layer chromatography]. Nahrung 10 273-274. (German)... 

Work on the determination of chlorinated insecticides has been almost exclusively in the area of gas chromatography using different types of detection systems, although a limited amount of work has been carried out using liquid chromatography and thin-layer chromatography. 

Sherma and Slobodien [107] determined chloropyrofos insecticide and its metabolite 3,5,6-trichloro-2-2-pyridinol in potable water at 5pg L 1 by thin layer chromatography. Pre-adsorbent silica gel layers were used for resolution and silver nitrate for detection prior to reflectance scanning. Recovery of 3,5,6-trichloro-2,2-pyridinol from water was 84%. Recovery of chloropyrifos from potable water was 87.5%. 

Thin-layer chromatography Is commonly employed to determine pigmentation of plants, using extracts of leaves. This method is also used to detect pesticides or insecticides in foods and has been instrumental in analysis of the dye composition of fibres from clothing in forensic investigations. 

Futagami K, Narazaki C, Kataoka Y, Shuto H, Oishi R. Application of high-performance thin-layer chromatography for the detection of organophosphorus insecticides in human serum after acute poisoning. J Chromatogr B 1997 704 369-73. 

Traditional analysis for cyclodiene insecticides involves their extraction from rendered adipose fat followed by gas or thin layer chromatography and detection by electron capture or mass spectrometry (6z. 1). These methods are time consuming, require sophisticated equipment and highly trained personnel. Because of the persistence of cyclodiene insecti-... 

Helling et al. (1971), also working with a Chlorella (C. sorokiniana), detected the movement of 11 triazine herbicides, nine phenylureas, and 13 other miscellaneous herbicides, together with two methylcarbamate insecticides, in a thin layer of soil supported on a chromatography plate. The Chlorella suspension was aspirated onto the soil-covered plate, which was then incubated at 100% relative humidity zones of pesticide movement were visible within 24 to 48 hr by algal inhibition. The authors foimd this procedure to be adaptable to detection of movement of many other herbicides and some insecticides and fungicides. 

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