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Thermoresponsive cell culture surface

Nanostructure of Thermoresponsive Cell Culture Surfaces Facilitates... [Pg.97]

This chapter focuses on the characteristics of thermoresponsive cell culture surfaces for preparing cell sheets and the applications for cell sheet-based regenerative therapies. Designing new types of thermoresponsive cell culture surfaces as the next generation for creating further functional and complex tissues is also described. [Pg.98]

FIGURE 3.2.3 Cell sheet fabrication using thermoresponsive cell culture surface. By changing temperature from 37°C to 20 C, cultured cells were harvested as a contiguous cell sheet. [Pg.100]

ECM-Mimicking Nanostructure on Thermoresponsive Cell Culture Surfaces for Creating Functional Cell Sheets... [Pg.102]

Table 3.2.2 ECM-Mimicking Thermoresponsive Cell Culture Surfaces... Table 3.2.2 ECM-Mimicking Thermoresponsive Cell Culture Surfaces...
Moreover, heparin-functionalized thermoresponsive cell culture surfaces have an affinity interaction with heparin-binding EGF-like growth factor (HB-EGF), which is a potent mitogen for hepatocytes [41]. An HB-EGF-bound heparin-functionalized thermoresponsive surface was utilized for creating hepatocyte sheets with maintaining hepatic functions during cultivation [42]. The addition of HB-EGF in the cell culture media was es-sentialfor the survival of hepatocytes. When the medium contained less than 10 ng/cm of soluble HB-EGF, the hepatocytes were not able to adhere and form their cell sheets. Hepatocytes adhered well and formed their sheets on the HB-EGF-bound heparin-functionalized... [Pg.104]

Most interesting, however, are thermoresponsive polymer phase transitions in aqueous solutions since this phenomenon provides high potential for biomedical applications, such as drug dehvery and switchable synthetic cell culture surfaces (de las Heras Alarcon et al, 2005 Schmaljohann, 2006 Ward and Theoni, 2011). The remainder of this chapter will focus on such temperature-responsive polymers in aqueous solution, by discussing basic principles (Section 2.2) and key types of temperature-responsive polymers (Section 2.3) as well as selected applications (Section 2.4). [Pg.18]

Takezawa T, Mori Y, Yoshizato K (1990) Cell-culture on a thermoresponsive polymer surface. Bio-Technology 8 854-856... [Pg.265]

Tsuda Y, Kikuchi A, Yamato M et al (2004) Control of cell adhesion and detachment using temperature and thermoresponsive copolymer grafted culture surfaces. J Biomed Mater Res A 69 70-78... [Pg.265]

Brun-Graeppi, A. K. A. S., Richard, C., Bessodes, M., Scherman, D., Merten, O.-W. (2010). Thermoresponsive surfaces for cell culture and enzyme-free cell detachment. Progress in Polymer Science, 35, 1311-1324. [Pg.32]

Sakai, H., Okano, T., Yamada, N., Sakurai, Y. (1996). Thermoresponsive polymer surface for cell culture analysis of the surface and control of the cell attachment/detachment. In N. Ogata, S. Kim, J. Feijen, T. Okano (Eds.), Advanced biomaterials in biomedical engineering and drug delivery systems. Tokyo Springer. [Pg.231]

Loh, X. J., Gong, J., Sakuragi, M., Kitajima, T., Liu, M., Li, J., et al. (2009b). Surface coating with a thermoresponsive copolymer for the culture and non-enzymatic recovery of mouse embryonic stem cells. Macromolecular Bioscience, 9, 1069-1079. [Pg.229]


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See also in sourсe #XX -- [ Pg.98 ]




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