Thermoresponsive surfaces

Yoshioka H, Mikami M, Mori Y, Tsuchida E (1993) Preparation of thermoresponsive surfaces using polyvinylchloride-graft-poly(A-isopropylacrylamide). Polym Adv Technol 4 519-521 Yu H, Grainger DW (1993) Thermosensitive swelling behavior in cross.linked N-isopropylacry-lamide networks-cationic, anionic, and ampholytic hydrogels. J Appl Polym Sci 49 1553-1563... 

D. Cunliffe, C. de las Heras Alarcon, V. Peters, J.R. Smith, C. Alexander, Thermoresponsive surface-grafted poly (N-isopropylacrylamide) copolymers effect of phase transitions on protein and bacterial attachment, Langmuir 19 (2003) 2888-2899. 

FIGURE 3.2.4 ECM-mimicking thermoresponsive surface containing (a) RGD peptides and (b) heparin molecules. 

Moreover, heparin-functionalized thermoresponsive cell culture surfaces have an affinity interaction with heparin-binding EGF-like growth factor (HB-EGF), which is a potent mitogen for hepatocytes [41]. An HB-EGF-bound heparin-functionalized thermoresponsive surface was utilized for creating hepatocyte sheets with maintaining hepatic functions during cultivation [42]. The addition of HB-EGF in the cell culture media was es-sentialfor the survival of hepatocytes. When the medium contained less than 10 ng/cm of soluble HB-EGF, the hepatocytes were not able to adhere and form their cell sheets. Hepatocytes adhered well and formed their sheets on the HB-EGF-bound heparin-functionalized... 

Brun-Graeppi, A. K. A. S., Richard, C., Bessodes, M., Scherman, D., Merten, O.-W. (2010). Thermoresponsive surfaces for cell culture and enzyme-free cell detachment. Progress in Polymer Science, 35, 1311-1324. 

Moran, M. T., Carroll, W. M., Gorelov, A., Rochev, Y. (2007a). Intact endothelial cell sheet harvesting from thermoresponsive surfaces coated with cell adhesion promoters. Journai of the Royai Society Interface, 4, 1151-1157. 

Cunliffe, D., Heras Alarcon, C., Peters, V., Smith, J. R., and Alexander, C. 2003. Thermoresponsive surface-grafted poly(N-isopropylacrylamide) copolymers Effect of phase transitions on protein and bacterial attachment. 

Tsuda Y, Kikuchi A, Yamato M, Nakao A, Sakurai Y, Umezu M, Okano T (2005) The use of patterned dual thermoresponsive surfaces for collective recovery as co-cultured cell sheets. Biomaterials 26(14) 1885-1893... 

S.4 Control of the desorption of cells from a thermoresponsive surface... 

Thermoineversible gels, 189 Thermoresponsive chromatography, 136-9 Thermoresponsive gels, 68-71 Thermoresponsive polymers, fixation, 185-7 Thermoresponsive surface... 

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