The Nature and Size Distribution of Milk Fat Globules

Fat in milk is present predominantly in spherical droplets ranging from 0.2 to 15 pm in diameter bovine milk typically contains 1010 fat 

Huppertz and A.L. Kelly Department of Food and Nutritional Sciences, University College, Cork, Ireland. 

Advanced Dairy Chemistry, Volume 2 Lipids, 3rd edition. 

Avoidance of interference of other milk constituents with measurements is also of importance for example, dissociation of casein micelles by calcium-chelating agents, such as trisodium citrate or ethylenediamine tetra-acetic acid (EDTA), may used to avoid interference of the micelles in particle size measurement, while clusters of fat globules can be disrupted by adding a low level of sodium dodecyl sulphate (SDS). 

Several parameters can be used to express the mean size of the milk fat globules. These parameters are derived from the so-called moments of the size distribution function the th moment of the distribution function (Sn) is equal to  

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