Tetracycline resistant genes

A number of laboratories have studied the effect of methylation on nucleosome placement and/or positioning. Methylation of several sequences did not seem to affect this parameter sequences from the tetracycline resistance gene [165], Alu repeats from human DNA [166], 5S rDNA from X. borealis [164]. On the other hand, nucleosomes were excluded from a specific region in the /6-globin gene, when methylated [167], indicating the importance of the DNA sequence. Earlier data by Keshet et al. [118] showed disappearance of nucleosome-free regions (i.e., formation of nucleosomes) in methylated promoters in vivo. This effect, however, may be only indirectly connected to the methylation status of the promoters. 

Peak N, Knapp CW, Yang RK et al (2007) Abundance of six tetracycline resistance genes in waste water lagoons at cattle feedlots with different antibiotic use strategies. Environ Microbiol 9(1) 143-151... 

Auerbach EA, Seyfried EE, McMahon KD (2007) Tetracycline resistance genes in activated sludge waste water treatment plants. Water Res 41(5) 1143-1151... 

Hillen W, Gatz C, Altschmied L et al (1983) Control of expression of the TnlO-encoded tetracycline resistance genes. Equilibrium and kinetic investigation of the regulatory reaction. J Mol Biol 169 707-721... 

Cbee-Sanford J.C., R.I. Aminov, I.J. Krapac, N. Garrigues-Jeanjean, and R.I. Mackie (2001). Occurrence and diversity of tetracycline resistance genes in lagoons and groundwater under-l)dng two swine production facilities. Applied and Environmental Microbiology 67 1494-1502. 

FIGURE 9-4 The constructed E. coli plasmid pBR322. Note the location of some important restriction sites—for Pstl, fcoRI, Ba/nHI, SalI, and PvuII ampicillin- and tetracycline-resistance genes and the replication origin (ori). Constructed in 1977, this was one of the early plasmids designed expressly for cloning in E. coli. 

Figure 26-7 Genetic map of cloning plasmids pBR322 and pUC18. Abbreviations ori, origin of replication Amp1, ampicillin resistance gene Tet1, tetracycline resistance gene. Other abbreviations are for sites cleaved by specific restriction endonucleases, a few of which are defined in Table 26-2. The nucleotide sequence numbers and directions of transcription are also indicated. Reproduced by permission of Amersham Pharmacia Biotech Inc.

Fig. 7.11 Schematic diagram of foreign gene expression in plant. P promoter, T terminator, pGA482 plant cloning vector, Km kanamycin resistance gene, Tc tetracycline resistance gene.

Tetracyclines have been extensively used in animal feeds to enhance growth. This practice has contributed to the spread of tetracycline resistance among enteric bacteria and of plasmids that encode tetracycline resistance genes. 

Feedback coupling was achieved by introducing a T7 promoter upstream of the tetracycline resistance gene (tet). This resulted in the exclusive dependence of re-... 

Fig. 4.3.2. Scheme for the genetic selection of an error-prone RNA polymerase variant based on the reversion of antibiotic resistance. The selection plasmid carries a mutant tetracycline resistance gene under the control of a T7 promoter. This construct renders the host cell sensitive to tetracycline (tet) unless an... 

The pBR322 plasmid carries genes that confer resistance to both ampicillin and tetracycline. The Bam II site lies within the tetracycline resistance gene. If the gene of interest has been inserted into... 

Cells that do not grow on this plate have an insertion in the tetracycline resistance gene. 

Figure V-18 Insertional inactivation of the tetracycline resistance gene following cloning in pBR322.

Roberts MC. Update on acquired tetracycline resistance genes. 

Speer BS, Salyers AA. Novel aerobic tetracycline resistance gene 75. that chemically modifies tetracycline. 1. Bacteriol. 1989 171(1) 148-153. 

The fd-tet phage genome contains a tetracycline resistance gene. Thus, always amplify E. eoli cells infected with phage in the presence of both kanamycin and tetracycline antibiotics (7). 

Specific primers to tetracycline resistance gene (see Note 4)... 

Palva, A. et al. (1990) Nucleotide sequence of the tetracycline resistance gene of pBC16 om Bacillus cereus. Nucleic Acids Res., 18 (6), 1635. 

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