Terminal determination

For carboxyl terminal determination of peptides by means of CDI the terminal carboxylic acid group of the peptide is selectively reduced with sodium dihydrobis(2-methoxy-ethoxy)aluminate to an alcohol. Subsequent conversion of the amino alcohol moiety with CDI yields an 7V-acyl-2-oxazolidone derivative, from which the oxazolidone unit can be easily removed and characterized.[56]... 

Furthermore, the maximum specific growth rate, p7/, can be determined where substrate-nonlimited conditions exist, and the saturation constant, KSw, can be found where such conditions are just being terminated. Determination of these two process parameter follows the principle that is described in Section 7.2.1. 

The amino acid analysis (Table I) strongly supports the value of 31,000 for the molecular weight of DNase I. This value has been accepted in Stockholm (34) and in New York 35), making all previously reported values obsolete [see reviews (10, 26, 27, 30) ]. The values obtained (Table II) by ultracentrifugation methods sedimentation diffusion and approach to equilibrium closely agree with the value of 31,000 based on N-terminal determination. 

Determine cellular fate/ biodistribution and persistence (cell migration/trafficking, engraftment, differentiation, cell phenotype, fusion, tissue integration, proliferation, survival). Use of noninvasive (nonterminal) imaging modalities and special histological stains (terminal). Determine presence of ectopic foci. Justify multiple time points (e.g., 1 week, 1 month, 3 months, 6 months or 12 months). 

Owing to the snccess of cobalt(III)-mediated hydrolysis of amino acid esters, the next step was to examine how these complexes reacted with peptides. If similar hydrolytic results could be obtained with peptides, then one of the potential uses of cobalt(III) complexes would be in the N-terminal determination and seqnential analysis of polypeptides. This area has been investigated by several gronps. Peptides... 

Figure 5.2 Liberation of C-terminal amino acids from peptide by carboxypeptidase An example of the C-Terminal determination uses carboxypeptidase which liberates amino acids sequentially from peptide-LeuAspPhecoon-...

Results from the conjugation of the derivative of glucose were compared with those obtained by conjugation of the same derivative of the terminal determinant of the O-PS of V. cholerae, serotype Ol (Ogawa). The most important conclusion of the study was that efficiency of conjugation is concentration dependent and that the nature of the carbohydrate does not substantially affect the conjugation within a comparable size of ligands. ... 

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