Template-assembled synthetic proteins TASPs

Mutter, M. and Vuileumier, S. (1989) A Chemical Approach to protein Design-Template-Assembled Synthetic Proteins (TASP). Angewandte Chemie, 28, 535-676. 

Scheme 1 The Concept of Template-Assembled Synthetic Proteins (TASP) Topological Templates Induce Folding of Covalently Attached Peptide Blocks into Predetermined Packing Arrangements 5 13 141...

The recent work on template assembled synthetic proteins (TASP) is worthy of further mention [5, 6]. These are synthetic proteins in which short characteristic structural elements (e.g. a-helices, P-shcets) are brought together in a... 

Fig. 1. Template assembled synthetic proteins (TASP) hypothetical folding path. (Adapted from [5])...

G. Tuchscherer, Template assembled synthetic proteins (tasp) as functional mimetics of proteins, Angew. Chem., lnt. Ed. Engl. 1996, 35, 1482. 

P.E. Dawson, S.B.H. Kent, Convenient total synthesis of a 4-helix template-assembled synthetic protein (TASP) molecule by chemoselective ligation,/. Am. Chem. Soc. 1993, 115, 7263. 

In Fmoc chemistry, Lys and Om are conveniently blocked with the Boc protecting group [155]. For more temporary protection, the l-(4,4 -dimethyl-2,6-dioxocyclohexylidene)ethyl (Dde) protecting group is useful in the preparation of branched peptides, multiple antigenic peptides (MAPs), cyclic peptides, template-assembled synthetic proteins (TASPs), and templates for combinatorial chemistry [156-161]. Dde is quasi-orthogonal because the use of dilute hydrazine to cleave Dde partially removes the A -... 

Morgan CJ, Gelfand M, Atreya C, Miranker AD (2001) Kidney dialysis-assodated amyloidosis a molecular role for copper in fiber formation. J Mol Biol 309 339-345 Murphy RM (2002) Peptide aggregation in neurodegenerative disease. Annu Rev Biomed Eng 4 155-174 Mutter M, Vuilleumier S (1989) A chemical approach to protein design— template-assembled synthetic proteins (TASPs). Angew Chem Int Ed Engl 28 535-554... 

Wong AK, Jacobsen MP, Winzor DJ, Fairlie DP (1998) Template assembled synthetic proteins (TASPs). Are template size, shape, and directionality important in formation of four-heUx bundles J Am Chem Soc 120 3836-3841... 

Leufgen et al. [82] reported the investigation of a template-assembled synthetic protein (TASP) conformation and its orientation in self-assembled monolayers (SAM) by means of TOF-SIMS. In this study, both the presence and molecular orientation of the TASP molecule can be monitored by TOF-SIMS, and furthermore, the influence of self-assembly protocols on the TASP molecular surface orientation can be studied by TOF-SIMS, although Fourier transform infrared spectroscopy (FTIR) and surface plasmon resonance (SPR) fail in this case. 

Futaki S, Sogawa K, Maruyama J, Asahara T, Niwa M (1997) Preparation of peptide thioesters using Fmoc-solid-phase peptide synthesis and its application to the construction of a template-assembled synthetic protein (TASP). Tetrahedron Lett 38 6237-6240... 

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