Technetium antibody labelling

However, problems are encountered in production of rhenium radionuclides and work is being done to increase the yields of the radionuclides to meet urgent demands for their use in therapy. Moreover, rhenium is not as reactive as technetium. This situation makes rhenium chemistry somewhat specific - optimum conditions in the preparation of rhenium complexes or in antibody labeling using bifunctional ligands must be identified. 

Ranadive, G.N., Rosenzweig, H.S., Epperly, M.W., Seskey, T., and Bloomer, W.D. (1993) A new method of technetium-99m labeling of monoclonal antibodies through sugar residues, a study with TAG-72 specific CC-49 antibody. Nucl. Med. Biol. 20, 719-726. 

Thakur, M.L., and DeFulvio, J.D. (1991) Technetium-99m labeled monoclonal antibodies for immuno-scintigraphy. J. Immunol. Metb. 137, 217-224. 

Arcitumomab is a murine F(ab )2 fragment from an anti-carcinoembryonic antigen (CEA) antibody labeled with technetium 99m... 

HTia receptor and the alphal-adrenergic receptor. Nucl Med Biol 29 375-387 Hnatowich DJ, Nakamura K (2004) Antisense targeting in cell culture with radiolabelled DNAs - a brief review of recent progress. Ann of Nucl Med 18 363-368 Horn RK, Katzenellenbogen JA (1997) Technetium-99m-labelled receptor-specific small-molecule radiopharmaceuticals recent developments and encouraging results. Nucl Med Biol 24 485-498 Iwasaki T, Iwasaki I, Aihara Y et al (2001) Immunoscintigraphy of aortic dissection with " Tc-la-belled murine anti-smooth muscle myosin monoclonal antibody in rats. J Nucl Med 42 130-137... 

Becker W, Bair J, Behr TM, Repp R, Streckenbach H, Beck H, Gramatzki M, Winship MJ, Goldenberg DM, Wolf F (1994) Detection of soft-tissue and osteomyeltis using a technetium-99m labeled anti-granulocyte monoclonal antibody fragment. J Nucl Med 35 1436-1443... 

Mather S J, Ellison D (1990). Reduction-mediated technetium-99m labeling of monoclonal antibodies. J. Nucl. Med. 31 692-697. 

Stalteri M A, Mather S J (1996). Technetium-99m labelling of the anti-tumor antibody PR1A3 by photoactivation. Eur. J. Nucl. Med. 23 178-187. 

Antibody labelling methods are normally described either as direct in which the technetium is complexed directly by the atoms present in the protein chain... 

Reduction-mediated technetium-99m labelling of antibodies with 2-mercaptoethanol... 

Indium-lll labelling of antibodies using cyclic DTPA anhydride 221 Reduction-mediated technetium-99m labelling of antibodies with 2-mercaiJtoethanol 226... 

N-donor groups in the form of e-amino groups of lysine as nonspecific binding sites for technetium may play an undesired role in Tc-labelling of monoclonal antibodies for tumour imaging [51]. Nonspecifically bound Tc has a poor in vivo stability and appears to increase the undesired liver uptake and reduces tumour uptake. The metal oxidation state and coordination is not yet known. 

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