RNA Targeting Antisense

Not only eukaryotic cells but also bacteria have successfully been targeted by PNA anhsense strategies. Thus it has been shown that PNA complementary to ribosomal RNA or mRNA encoding an essential fatty acid biosynthesis protein, effectively kills E. coli. Furthermore, it has been shown that PNA directed to the start codon of the y -lactamase gene re-sensitized otherwise resistant E. coli to the antibiohc ampiciUin [64—66]. Conjugating a simple transporter peptide to the PNA increased the potency significantly, and an even more potent antibacterial PNA [Pg.160]

PNA Target Method Modification Cell type/line Assay Reference [Pg.161]

21-mer Galanin receptor (ORF) Direct delivery Peptide conjugate (penetratin / transportan ) Human melanoma Bowes Receptor activity/pro-tein level (Western blot) [56] [Pg.161]

16-mer Pre-pro-05 ocin Direct delivery Peptide conjugate (ret-ro-inverso penetratin ) Primary rat neurons mRNA level (RT-PCR) 1 mmun ocyt ol ogy [55] [Pg.161]

14-mer (homo-pyrimidine) Nitric oxide synthase Direct delivery PNA peptide conjugate (Phe-Leu)3 Mouse macrophage RWA264.7 En2yme activity [125] [Pg.161]

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