Systems for Bioseparations

KIN King, R.S., Blanch, H.W., and Prausnitz, J.M., Molecular thermodynamics of aqueous two-phase systems for bioseparations, 34,1585,1988. 

F. Tjerneld and G. Johansson, Aqueous two-phase systems for biotechnical use, Bioseparation 1990, 1, 255-263. 

Mattiasson B, Ling TGI (1987) Extraction in aqueous two-phase systems for biotechnology. In Verrall MS, Hudson MJ (eds) Bioseparation for biotechnology. Ellis Horwood, Chichester, p 270... 

Liquid-liquid partitioning is a convenient and often economical method for bioseparations. L. Gu (personal communication, 1999) has shown that an acetonitrile-water system can be used for separation of proteins. This system partitions into two phases under subzero temperatures with the top phase containing more acetonitrile and water. The low temperature and the presence of water in both phases help reduce protein denaturation. An added advantage is that sample solution can be directly applied to reversed-phase high-performance liquid chromatography (HPLC) for further purification. Aqueous liquid-liquid partitioning is likely to remain an attractive choice for the separation of proteins, and exploration of new systems will continue. 

Szlag DC, Giuhano A, and Snyder SM, A low-cost aqueous two phase system for affinity extraction. In Hamel JFP, Hunter JB, and Sikdar SK, eds. Doivnstream Processing and Bioseparation, Recovery and Purification of Biological Products. ACS Symp. Ser. 419, ACS Press, Washington, 1990 pp. 71-86. 

V. Derbyshire, M. Belfort, A genetic system yields self-cleaving inteins for bioseparations, Nat. Biotechnol. 1999, 17,889-892. 

The tubing connecting the sample introduction unit, column, and detector consists of small-volume stainless steel capillaries with an inside diameter of 0.25 mm and an outer diameter of 1.25 mm (1/16 inch). For bioseparations that are sensitive to stainless steel, tubing made from polyethere-therketone (PEEK) is recommended. All connecting tubing, e.specially between sample introduction system and column, should have a small vol-... 

Diamond, A., Hsu, J. (1992). Aqueous two-phase systems for biomolecule separation. Bioseparation. Springer. 

The problems of bio-functional polymers were diseussed on the 4 session. This session included 6 lectures. The speakers gave information about biofunctional dendritic architectures, biocompatible and bioactive polymers containing saccharide fimctionality, design and mechanisms of antimicrobial polymers, control of protein adsorption on functionalized electrospun fibers, microcapsules and nanoparticles for controlled delivery and repair, smart nanocarriers for bioseparation and responsive drug delivery systems. 

Utilization of nanoparticle systems for enhancing a phenomenon or process, such as chemical reactions, nano-elcctronics, nano-ionics, magnetic processes, optical processes, heat transfer, bioseparation, bio and chemical reactivity. 

At present, the purification by chromatographic processes is the most powerful high-resolution bioseparation technique for many different products from the laboratory to the industrial scale. In this context, continuous simulated moving bed (SMB) systems are of increasing interest for the purification of pharmaceuticals or specialty chemicals (racemic mixtures, proteins, organic acids, etc.).This is particularly due to the typical advantages of SMB-systems, such as reduction of solvent consumption, increase in productivity and purity obtained as well as in investment costs in comparison to conventional batch elution chromatography [1]. 

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