Synthesis transfection

Keywords Applications Cytotoxicity profile Gene delivery Hyperbranched polyamines Poly(amido amine) Poly(amido ester) Poly(ethylene imine) Polyglycerol amines Structure-property dependence Synthesis Transfection efficiency... 

Subsequently, similar experiments were done with viral nucleic acids. The pure viral nucleic acid, when added to cells, led to the synthesis of complete virus particles the protein coat was not required. This process is called transfection. More recently, DNA has been used in cell-free extracts to program the synthesis of RNA that functions as the template for the synthesis of proteins characteristic of the DNA... 

Sequential hydroformylation/reductive amination of dendritic perallylated polyglycerols with various amines in a one-pot procedure to give dendritic polyamines in high yields (73-99%). Furthermore, the use of protected amines provides reactive core-shell-type architectures after deprotection. These soluble but membrane filterable multifunctional dendritic polyamines are of high interest as reagents in synthesis or as supports in homogeneous catalysis as well as nonviral vectors for DNA-transfection (Scheme 18) [65]. 

Fichert T, Regelin A, Massing U. Synthesis and transfection properties of novel non-toxic monocationic lipids. Variation of lipid anchor, spacer and head group structure. Bioorg Med Chem Lett 2000 10(8) 787-791. 

Lenssen K, Jantscheff P, von Kiedrowski G, Massing U. Combinatorial synthesis of new cationic lipids and high-throughput screening of their transfection properties. Chembiochemisty 2002 3(9) 852-858. 

Lynn DM, Anderson DG, Putnam D, Langer R (2001) Accelerated discovery of synthetic transfection vectors parallel synthesis and screening of a degradable polymer library. J Am Chem Soc 123 8155-8156... 

J.X. Yu, P. Otten, Z. Ma, W. Cui, L. Liu, R.P. Mason, A novel NMR platform for detecting gene transfection Synthesis and evaluation of fluorinated phenyl - -Galactosides with potential application for assessing LacZ gene expression, Bioconjug. Chem. 15 (2004) 1334-1341. 

In this chapter, the synthesis and structure of commonly used cationic lipids for gene delivery and the influence of their physicochemical properties on transfection activity will be described. 

The synthesis of DOTMA was performed first using D-mannitol-3,4-acetonide as a starting material (Figure 15.2). This tedious procedure was improved for the synthesis of a variety of DOTMA related cationic lipids (Bennett et al., 1995). Their synthesis was accomplished using a simplified synthetic approach also suitable for the synthesis of DOTMA (Figure 15.3). This procedure allowed access to quaternary ammonium esters with less toxicity and to a variety of quaternary ammonium salts (Figure 15. 4) with improved transfection efficacy. 

Gaucheron, J., Santaella, C. and Vierling, P. (2001) Highly fluorinated lipospermines for gene transfer Synthesis and evaluation of their in vitro transfection efficiency. Bioconjug. Chem., 12, 114-128. 

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