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SynChropak columns

Each SynChropak column is tested chromatographically to assure that it has been packed according to specifications. For SynChropak GPC columns, a mixture of a high molecular weight DNA and glycyltyrosine, a dipeptide, is used to evaluate internal volume and efficiency. The mobile phase used for the test is 0.1 M potassium phosphate, pH 7, and the flow rate is 0.5 ml/min for 4.6-mm i.d. columns. Minimum plate count values and operational flow rates are listed in Table 10.4 for 4.6-mm i.d. columns of all supports and the various diameters of the SynChropak GPC 100 columns. [Pg.314]

Scientific (Northbrook, IL) contain a silica support with a -y-glycidoxypropylsi-lane-bonded phase to minimize interaction with anionic and neutral polymers. The columns come in five different pore sizes ranging from 100 to 4000 A. The packing material has a diameter from 5 to 10 /cm and yields in excess of 10,000 plate counts. With a rigid silica packing material, the columns can withstand high pressure (maximum of 3000 psi) and can be used under a variety of salt and/or buffered conditions. A mobile phase above pH 8, however, will dissolve the silica support of the column (21). A summary of the experimental conditions used for Synchropak columns is described in Table 20.8. [Pg.572]

TABLE 20.8 Summary of Experimental Conditions Used with Synchropak Columns... [Pg.572]

FIGURE 20.7 DRI chromatograms of anionic polymers using Synchropak columns with an aqueous mobile phase of 0.10 N sodium nitrate. [Pg.573]

Synchropak columns are very useful for characterizing hydrophilic, anionic, and nonionic, water-soluble polymers, CATSEC columns work best for characterizing cationic polymers utilizing both light scattering and/or differential viscometry detection over a wide range of molecular weights. [Pg.580]

A 25 cm X 4.6 mm ID long 300A SynChropak column was used to evaluate temperature effects. Injections were made with 5% D2O and 1.3 mg/ml glucose solutions. D2O gave a negative refractive index response. [Pg.210]

TABLE II. ELUTION CHARACTERISTICS OF D2O AND GLUCOSE ON SYNCHROPAK COLUMNS ... [Pg.211]

S in/min lOOA Synchropak column, other conditions. See Table II for... [Pg.214]

The retention times of glucose and D2O as a function of column temperature using a 300A SynChropak column are in Table VII. [Pg.215]

TABLE VII. EFFECT OF COLUMN TEMPERATURE ON THE ELUTION TIME OF D2O AND GLUCOSE USING A 300A SYNCHROPAK COLUMN ... [Pg.215]

SynChropak GPC supports were introduced in 1978 as the first commercial columns for high-performance liquid chromatography of proteins. SynChropak GPC columns were based on research developed by Fred Regnier and coworkers in 1976 (1,2). The first columns were only available in 10-yu,m particles with a 100-A pore diameter, but as silica technology advanced, the range of available pore diameters increased and 5-yu,m particle diameters became available. SynChropak GPC and CATSEC occasionally were prepared on larger particles on a custom basis, but generally these products have been intended for analytical applications. [Pg.305]

For proteins, the most useful columns are those with pores of 100-500 A, as seen in Fig. 10.2, because most proteins elute on the linear portions of the calibration curves. Figure 10.5 illustrates an analysis of a protein mixture on SynChropak GPC100. Small peptides can be analyzed on the 50-A SynChro-pak GPC Peptide column with appropriate mobile-phase modifications. Many peptides have poor solubility in mobile phases standardly used for protein analysis, as discussed later in this chapter. [Pg.308]

When cationic polymers are run on SynChropak CATSEC columns, the calibration curves, as shown in Fig. 10.4, are not identical to those produced... [Pg.308]

FIGURE 10.2 Calibration curves for proteins on SynChropak GPC columns. Mobile phase 0.1 M potassium phosphate, pH 7. (From MICRA Scientific, Inc., with permission.)... [Pg.308]

SynChropak GPC and CATSEC columns are packed in either stainless steel or PEEK columns with standard inverted fittings so that they readily connect to most instruments. Stainless-steel columns are available in 2.1, 4.6, 7.8, 10,... [Pg.310]

SynChropak CATSEC columns are evaluated similarly using a polyvinyl-pyridine standard of molecular weight 600,000 and cytidine. The mobile phase is 0.1 % trifluoroacetic (TEA) acid containing 0.2 M sodium chloride. Minimum plate counts are listed in Table 10.4. [Pg.314]

For many proteins, a simple buffer such as 0.1M phosphate, pH 7, produces excellent separations on SynChropak GPC columns. Generally, minimal interaction is achieved when the ionic strength is 0.05-0.2 M. To prevent denatur-ation or deactivation of proteins, the pH is generally kept near neutrality. For denatured proteins, 0.1% sodium dodecyl sulfate (SDS) in 0.1 M sodium phosphate, pH 7, is recommended. [Pg.315]


See other pages where SynChropak columns is mentioned: [Pg.571]    [Pg.572]    [Pg.572]    [Pg.574]    [Pg.574]    [Pg.576]    [Pg.209]    [Pg.210]    [Pg.187]    [Pg.571]    [Pg.572]    [Pg.572]    [Pg.574]    [Pg.574]    [Pg.576]    [Pg.209]    [Pg.210]    [Pg.187]    [Pg.305]    [Pg.305]    [Pg.306]    [Pg.307]    [Pg.307]    [Pg.308]    [Pg.311]    [Pg.312]    [Pg.313]    [Pg.314]    [Pg.315]    [Pg.317]    [Pg.318]    [Pg.319]   


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