Elution volumes SynChropak columns

Figure 1. Influence of flow rate on elution volume of D2O and glucose. The column was a SynChropak lOOA column. See Table II for conditions.

Figure 3 Nonspecific interactions in SEC. Column SynChropak GPC 60 (300 X 7.8 mm ID SynChrom, Lafayette, IN) Mobile phase 0.1% aqueous TFA containing either 10 mM KCI (A), 50 mM KCI (B), or 200 mM KCI (C) flow rate, 1 mL/min temperature, 26 C. Peptides 1,2, and 5 are synthetic peptide standards containing 10, 20, and 50 residues, respectively (sequences shown in Table 1). The arrow denotes the elution time for the total permeation volume of the column.

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