Dilute suspension

Add 50 pL of a log-phase bacterial suspension, diluted to approximately 1.5 x 106 cells/mL, in a microfuge tube and then add 100-x pL of liquid medium (e.g., MH broth), where x corresponds to the volume of peptide to be assayed (x = 0 in the control tubes). Use the PRP at a bactericidal concentration for the parental wild-type strain (HB101). Different PRP peptide concentrations may be used (e.g. 5X MIC). 

The assay of the standard and unknown lysozyme should be performed with the same substrate suspension. In the assay a suspension diluted with phosphate buffer to an absorbance of 0,800 0.050 at 450 nm is used. 

A review of consequences of various forces acting on suspensions dilute suspensions at order 4> and [Pg.3]

Determine viability using trypan blue Place 0.5 mL of cell suspension (dilute cells to an approximate concentration of 1 x 105 to 2 x 105 cells per mL) in a screw cap test tube. Add 0.1 mL of 0.4% trypan blue stain. Mix thoroughly. Allow to stand for 5 min to 10 min at room temperature. Using a hemocytometer, under a microscope, determine the per cent ofnonviable (stained) and viable cells (see Note 7). 

Henderson, L.M. Johnson, C.E. Berardi, R.R. Stability of mesalamine in rectal suspension diluted with distilled water. Am.J.Hosp.Pharm., 1994, 51, 2955-2957... 

Sample preparation Shake, remove 2 mL of oral suspension, dilute to 40 mL with water, vortex 1 min, centrifuge at 2000 rpm for 10 min. Dilute a 100 (jiL aliquot of supernatant with 100 jjiL of 100 jjig/mL theophylline and add 800 p,L water, inject 20 p-L aliquot. 

Sample preparation Suspensions. Dilute 2 mL suspension to 1 L with EtOH, remove a... 

Sample preparation Tablets. Powder tablets, weigh out an amount equivalent to about 100 mg trimethoprim, suspend in MeOH, sonicate for 2 min, filter, dilute with MeOH to 250 p-g/mL. Suspensions. Dilute with MeOH to 250 p-g/mL, sonicate, filter. 

Sample preparation Tablets. Crush tablets, weigh out amount equivalent to about 40 mg trimethoprim, dissolve in EtOH, filter, make up to 200 mL with EtOH. Suspensions. Dilute 5 mL suspension to 100 mL with EtOH, filter. 

Sample preparation Capsules. Open five capsules, dissolve contents and shells in water, make up to 1 L with water, shake thoroughly, filter (0.2 p,m) an aliquot, dilute the filtrate 10-20-fold, inject an aliquot. Oral suspensions. Dilute 200-400-fold, filter, inject an aliquot of the filtrate. 

Sample preparation Suspensions. Dilute 2 mL suspension to 1 L with EtOH, remove a 2.5 mL aliquot and add it to 1 mL 1 m mL hydrocortisone in EtOH. Dilute this mixture to 50 mL with EtOH, inject an aliquot. Tablets. Grind tablets to a fine powder, stir with 50 mL EtOH, make up to 100 mL with EtOH, filter (Whatman No. 1 paper), reject the first 20 mL of the filtrate. Mix 2 mL of the filtrate with 1 mL 1 mg/mL hydrocortisone in EtOH, make this mixture up to 50 mL with EtOH, inject an aliquot. 

Table 24. Minimum Inhibition Concentrations (MIC) of iV-(3-chloroallyl)-hexaminium Chloride in Nutrient Agar and Minimum Lethal Concentrations (MLC) Determined through a Tube Suspension/Dilution Technique (Exposure Time 48 h)...

The size distribution of the liposomes is determined by dynamic light scattering (DLS) with a Dynapro apparatus (http //www.wyatt.com). DLS is a hydrodynamic method by which one determines the rate of diffusion of particles through the solvent. The hydrodynamic radius is defined as the radius of a theoretical hard sphere that diffuses with the same speed as the particle under examination. The measurement is performed at 25° and requires about 2 /ul of the extruded liposome suspension diluted in 18 /ul of liposome buffer (final lipid concentration in the range of 0.1 roM). Ten autocorrelation functions are sequentially measured, from which the size distribution of the liposome is determined using the Dynamics v5 software from Dynapro. A complete measurement takes a few minutes. Figure lA shows typical size distributions of extruded liposomes as determined by DLS. Figure IB shows how the actual hydrodynamic radius of the liposomes varies with the pore size of the polycarbonate filter. 

In all the above methods, care should be taken in sampling the suspension, which should cause as little disturbance as possible for the structure to be investigated. For example, when one investigates the flocculation of a concentrated suspension, dilution of the system for microscopic investigation may lead to break down of the floes and a false assessment is obtained. The same applies to examinations of the rheology of a concentrated suspension, since transfer of the system from its container to the rheometer may lead to a break down of the structure. 

Coprecipitation of the ZnAl-LDH and impregnation ofTiO onto thermally treated LDHs. The ZnAl-LDHs were synthesized by LS coprecipitation method at constant pH [80]. The precipitates were thermally treated -calcined and the newly formed LDH-based mixed oxides were used for the impregnation of TiO. The TiO suspension, diluted in base Na CO solution, was loaded onto thermally treated ZnAl-LDH powder and the excess water was removed in a rotary vacuum evaporator, dried and thermally activated at 500°C. 

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