Baker Suspension Dilution Method

The nanoemulsions in US. Patent 6,015, 832 (Tables 1 and 2 in patent) were tested for antimicrobial activity, using the same bacteria above, together with oleic fatty acid and caprylic fatty acid. The average particle size of the nanoemulsions in (US. Patent 6,015, 832) were smaller than reported in the patent, and 

Emulsions were prepared from caprylic and olive oil to evaluate the effect of caprylic acid as an antibacterial agent in an emulsion. Pure caprylic acid did not form a stable emulsion, but did so when dissolved in olive oil, and was stable in excess of 7 days. All other steps to emulsify the liquids were the same, and the results are shown in Table 4.5. Antibacterial activity is significant at 1% caprylic acid, but acceptable antibacterial activity is achieved after 

Note 2 TweenR surfactants (see Reference Section) were used consistently in the formulations for preparation of these emulsions 

Material Pseudomonas Proteus mirabilis aeruginosa Staphylococcus aureus 

Note 2 The standard disk diameter is 6 mm, an acceptable experimental value (zone of inhibition) is greater than the disc and can also be compared to chlorhexidine controls that are known to be effective antibacterial agents 

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