Surface plasmon resonance affinity biosensor

Fig. 3 Principle of surface plasmon resonance affinity biosensor...

The range of biosensors for the analysis of vitamins that use enzymes as the biorecognition elements is rather restricted. However, affinity biosensors have opened a new horizon in the field of vitamin analysis with surface plasmon resonance (SPR) biosensors at the forefront. Commercialization of optical SPR... 

Figure 7. Concept of affinity biosensor based on surface plasmon resonance.

However, the determination of affinity does not necessarily have to rely on labeled ligands. It is also possible with native ligands when using suitable detection methods, as for example nuclear magnetic resonance (NMR), surface plasmon resonance (SPR), acoustic biosensors or calorimetry [48, 49]. A particularly versatile and sensitive detection principle for the investigation of interactions between targets and native ligands is mass spectrometry [50]. 

Li, Y.L, et al. (2006) Reversible immobilization of proteins with streptavidin affinity tags on a surface plasmon resonance biosensor chip. Anal Bioanal Chem. 386, 1321-6. 

Scarano S, Mascini M, Turner APF, Minunni M (2010) Surface plasmon resonance imaging for affinity-based biosensors. Biosens Bioelectron 25 957-966... 

Mitsui K, Handa Y, Kajikawa K (2004) Optical fiber affinity biosensor based on localized surface plasmon resonance. Appl Phys Lett 85 4231 233... 

In Chap. 1 by J. Homola of this volume [1] surface plasmons were introduced as modes of dielectric/metal planar waveguides and their properties were estabhshed. It was demonstrated that the propagation constant of a surface plasmon is sensitive to variations in the refractive index at the surface of a metal film supporting the surface plasmon. In this chapter, it is shown how this phenomenon can be used to create a sensing device. The concept of optical sensors based on surface plasmons, commonly referred as to surface plasmon resonance (SPR) sensors, is described and the main approaches to SPR sensing are presented. In addition, the concept of affinity biosensors is introduced and the main performance characteristics of SPR biosensors are defined. 

Liedberg B. and Johansen K., Affinity biosensing based on surface plasmon resonance detection, in Biosensors Techniques and Protocols, ed. K. Rogers and A. Mulchandani (Totowa, NJ Humana Press 1998) Meth. BiotechnoL, 1, 31-54, 1998. 

Test and measurement sensors, pressure transducers, torque sensors, instrumentation, accelerometers, and displacement sensors An extensive array of instruments and systems for applications ranging from automotive R D, process and environmental monitoring, in vitro medical diagnostics Surface plasmon resonance biosensors for the determination of the affinity and kinetics of biomolecular interactions... 

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