Biosensor techniques

Here, we describe the design and preparation of antibody supramolecular complexes and their application to a highly sensitive detection method. The complex formation between antibodies (IgG) and multivalent antigens is investigated. When an antibody solution is mixed with divalent antigen, a linear or cyclic supramolecule forms [26-29]. With trivalent antigens, the antibody forms network structures. These supramolecular formations are utilized for the ampH-fication of detection signals on the biosensor techniques. 

The interaction between c Fusarium moniliforme and PGIP from Phaseolus vulgaris L. was investigated using a biosensor technique based on sur ce plasmon resonance (BIAlite). This new analytical system provides information on the strength and the kinetics of biomolecular interactions. 

Riedel K, Uthemann R (1994). SensorBSB - ein neuer mit Biosensoren gewonnener Summenparameter in der Abwasseranalytik. Wasserwirtsch-Wassertechn 2 35-38 Riedel K, Kunze G (1997) Rapid physiological characterization of microorganisms by biosensor technique.Microbiol Res 152 233-237... 

Beyersdorf-Radeck B, Riedel K, Karlson U, Bachmann TT, Schmid RD (1998) Screening of xenobiotic compounds degrading microorganisms using biosensor techniques. Microbiol Res 153 1-7... 

K. R. Rogers and A. Mulchandani. eds.. Affinity Biosensors. Techniques and Protocols (Totowa, NJ Humana Press, 1998) E. Palacek and M. Fojta, Detecting DNA Hybridization and Damage, Anal. Chem. 2001, 73, 75A. 

Very recently, a so-called surface plasmon resonance (SPR) biosensor technique has been developed that allows the differentiation and prediction of the degree of... 

K Riedel, In A. Mulchandemi emd K.R. Rogers (Eds.), Enzyme and Microbial Biosensors Techniques and Protocols. Humema Press, Totowa, NJ, 1998, pp. 199-224. 

Binding and/or unbinding of biomolecules at the active surface of an SPR biosensor is controlled by various mechanisms that result in variety of temporal profiles of the SPR biosensor response and in dependence on microenvironmental conditions. The determination of binding kinetics provides important new information about interacting molecules. This is commonly considered one of the greatest advantages of the SPR biosensor technique. Although in ideal cases an appropriate kinetic model of molecular interaction is able to completely describe the SPR biosensor response, in reality the influence of hydrodynamic conditions often has to be taken into account [1]. This chapter is devoted to molecular interaction models that correspond to the processes most frequently encoimtered at SPR biosensor surfaces. It also deals with hydrodynamic effects and their exact or approximate mathematical description. 

Affinity Biosensors Techniques and Protocols, edited by Kim R. Rogers and Ashok Mulchandam, 1998... 

Enzyme and Microbiai Biosensors Techniques and Protocols, edited by Ashok Mulchandani and Kim R. Rogers, 1998 5. Biopesticides f/se and Delivery, edited by Franklin R. Hall and Julius J. Menn, 1998 4. Naturai Products Isoiation, edited hy Richard J. P. Cannell, 1998... 

Liedberg B. and Johansen K., Affinity biosensing based on surface plasmon resonance detection, in Biosensors Techniques and Protocols, ed. K. Rogers and A. Mulchandani (Totowa, NJ Humana Press 1998) Meth. BiotechnoL, 1, 31-54, 1998. 

M. Murugesan, V. Murali Madhav, D. Jeyakumar. Rapid biochemical characterisation of polyurethane degrading fungi using amperometric biosensor technique. Bulletin of Electrochemistry, No. 15, pp. 452-470, 1999. 

M. Mascini, M. Minunni, G. G. GuUbault, R. Carter, in K. R. Rogers, A. Mulchandani, eds.. Affinity Biosensor. Techniques and Protocols, Humana Press Totowa, NJ (1998) Chapter 4. 

Electrochemical DNA biosensor techniques for the detection of microbiological and inherited diseases devoted to clinical analysis are presented dealing with past and novel developments in this chapter. For this purpose particular emphasis will be given to the most important approaches for electrochemical genosensing. 

As a laser-based optical biosensor technique, SPR can measure molecular binding events at a metal surface by detecting changes in the local refractive index. The depth probed at the metal-aqueous interface is typically 200 nm, making SPR a surface-sensitive technique ideal for studying interactions between immobihzed biological molecules and a solution-phase anal3he. 

For low-BOD samples, the kinetic mode has not been shown to be as sensitive as either of the conventional BOD methods or the steady-state biofilm method. Both biosensor techniques share with oxygen electrodes the danger of fouling of the biosensor membrane with oil. Because biofilm techniques allow rapid process control, they are most likely to find application in monitoring a consistent organic process waste. 

F. W. ScheUer, F. Schubert, Biosensor, Techniques and Instrumentation in Analytical Chemistry, Elsevier, Amsterdam, 1992, Vol. 11. 

E. W. Scheller, D. Pfeiffer, F. Usdat etal.. Enzyme biosensors based on oxygen detec-bon, in Methods in Biotechnology, Enzyme and Microbial Biosensors Techniques and Protocols (Eds. A. Mulchadani, K. R. Rogers), Humana press, Totowa, 1998, Vol. 6. 

Rogers KR (2006) Recent advances in biosensor techniques for environmental monitoring. Anal Chim... 

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