Surface plasmon imaging

Fig. 54. Experimental set-up for recording patterns at the electrode/electrolyte interface by means of surface plasmon imaging (top view g gas inlet).

There are a few other surface-sensitive characterization techniques that also rely on the use of lasers. For instance surface-plasmon resonance (SPR) measurements have been used to follow changes in surface optical properties as a fiinction of time as the sample is modified by, for instance, adsorption processes [ ]. SPR has proven usefiil to image adsorption patterns on surfaces as well [59]. 

Imaging of surface plasmon and ultrafast dynamics in gold nanorods by near-field microscopy. J. Phys. Chem. B, 108, 16344-16347. 

K. and Okamoto, H. (2005) Imaging and dispersion relations of surface plasmon... 

Brockman JM, Fmtos AG, Com RM (1999) A multistep chemical modification procedure to create DNA arrays on gold surfaces for the study of protein-DNA interactions with surface plasmon resonance imaging. J Am Chem Soc 121 8044-8051... 

Wegner GJ, Lee NJ, Marriott G, Com RM (2003) Fabrication of histidine-tagged fusion protein arrays for surface plasmon resonance imaging studies of protein-protein and protein-DNA interactions. Anal Chem 75 4740-4746... 

Kanda V, Kariuki JK, Harrison DJ, McDermott MT (2004) Label-free reading of microarray-based immunoassays with surface plasmon resonance imaging. Anal Chem 76 7257-7262... 

G. J. Wegner, H. J. Lee, and R. M. Com, "Characterization and Optimization of Peptide Arrays for the Study of Epitopeantihody Interactions Using Surface Plasmon Resonance imaging," Analytical Chemistry 74, 5161-5168 (2002). 

M. J. O Brien, V. H. Perez-Luna, S.R.J. Brueck, and G.P. Lopez, "A Surface plasmon Resonance Array Biosensor Based on Spectroscopic Imaging," Biosensors Bioelectronics 16, 97-108 (2001). 

A number of methods are available for the characterization and examination of SAMs as well as for the observation of the reactions with the immobilized biomolecules. Only some of these methods are mentioned briefly here. These include surface plasmon resonance (SPR) [46], quartz crystal microbalance (QCM) [47,48], ellipsometry [12,49], contact angle measurement [50], infrared spectroscopy (FT-IR) [51,52], Raman spectroscopy [53], scanning tunneling microscopy (STM) [54], atomic force microscopy (AFM) [55,56], sum frequency spectroscopy. X-ray photoelectron spectroscopy (XPS) [57, 58], surface acoustic wave and acoustic plate mode devices, confocal imaging and optical microscopy, low-angle X-ray reflectometry, electrochemical methods [59] and Raster electron microscopy [60]. 

Fig. 5 Surface plasmon microscopy images of a structured ultrathin PS film prepared by photopoiymerization through a mask A bare silicon appears dark, B polystyrene appears dark. (Reproduced with permission from [38] WUey)...

The 3-D NPH technology was applied to immobilize streptavidin (SA) onto Au-coated surface for surface plasmon resonance imaging (SPRi). By using 3-D NPH method, it was possible to improve the sensitivity of protein-protein interactions drastically comparing to the conventional protein immobilization method. 

Key words Surface plasmon resonance imaging technique. Small molecule arrays. Photoaffinity crosslinking, High-throughput screening. Sugar array. Signal normalization... 

Jordan, C. E. and Corn, R. M. (1997) Surface plasmon resonance imaging measurements of DNA hybridization adsorption and strepta-vidin/DNA multilayer formation at chemically modified gold surfaces. Anal. Chem. 69, 1449-1456. 

Nelson, B. P., Frutos, A. G., Brockman, J. M. and Corn, R. M. (1999) Near-infrared surface plasmon resonance measurements of ultrathin films. 1. Angle shift and SPR imaging experiments. Anal. Chem. 71, 3928-3934. 

Kyo, M., Yamamoto, T., Motohashi, H., Kamiya, T., Kuroita, T., Tanaka, T., Engel, J. D., Kawakami, B. and Yamamoto, M. (2004) Evaluation of MafG interaction with Maf recognition element arrays by surface plasmon resonance imaging technique. Genes Cells 9, 153-164. 

Inamori, K., Kyo, M., Nishiya, Y, Inoue, Y., Sonoda, T., Kinoshita, E., Koike, T. and Katayama, Y. (2005) Detection and quantification of on-chip phosphorylated peptides by surface plasmon resonance imaging techniques... 

Kim, M., Park, K., Jeong, E. J., Shin, Y. B., Chung, B. H. (2006) Surface plasmon resonance imaging analysis of protein-protein interactions using on-chip-expressed capture protein. Anal. Biochem. 351,298-304. 

J. M. Brockman. B. P. Nelson, and R. M. Com. Surface Plasmon Resonance Imaging Measurements of Ultrathin Organic Films, Annu. Rev. Phys. Chem. 2000,51, 41 J. Homola, Present and Future of Surface Plasmon Resonance Biosensors, AnaL Bioanal. Chem. 2003,377, 528 H. Q. Zhang,... 

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