Small molecule arrays

Key words Surface plasmon resonance imaging technique. Small molecule arrays. Photoaffinity crosslinking, High-throughput screening. Sugar array. Signal normalization... 

Most recently, we reported small molecule arrays on photoaffinity crosslinker coated gold surfaces (17). The small molecule arrays were fabricated by photoreaction, and then analyzed by SPR imaging technique. The small molecules don t have to be modified chemically for immobilization. The small molecules, which can interact with a target protein, can be screened by this methodology. Therefore, the integration of photoaffinity small molecule array and SPR imaging technique can be the first step of reverse chemical genetics. 

Kanoh, N., Kyo, M., Inamori, K., Ando, A., Asami, A., N ao, A. and Osada H. (2006) SPR imaging of photo-cross-linked small-molecule arrays on gold. Anal. Chem. 78, 2226-2230. 

Tab. 6.2.1. I immobilization reactions used for the preparation of DNA arrays, protein arrays, and small molecule arrays. 

Ramachandran N, Larson DN, Stark PR, Hainsworth E, LaBaer J. Emerging tools for real-time label-free detection of interactions on functional protein microarrays. FEES J. 2005 272 5412-5425. Kanoh N, Kyo M, Inamori K, Asami A, Nakao A, Osada H. SPR imaging of photo-cross-hnked small-molecule arrays on gold. Anal. Chem. 2006 78 2226-2230. 

Koehn, M., Wacker, R., Peters, C., Schroeder, H., Soulere, L., Breinbauer, R., Neimeyer, C. M., Waldmann, H. Staudinger ligation A new immobilization strategy for the preparation of small-molecule arrays. Angew. Chem., Int. Ed. Engl. 2003, 42, 5830-5834. 

In addition, protein chip arrays can be multifaceted by changing the capture ligand on the protein chip surface. For example, small molecule array is used to investigate smaU-molecule-protein interaction, AB array uses specific antibody as the capture ligand to study cellsignaling, and peptide array uses peptides as capture ligands to study protein—protein interaction. 

Kohn M et al. (2003) Staudinger ligation a new immobilization strategy for the preparation of small-molecule arrays. Angew Chem Int Ed 42 5830-5834... 

Kanoh N et al. (2006) SPR imaging of photo-cross-linked small-molecule arrays on gold. Anal Chem 78 2226-2230... 

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