Surface plasmon field-enhanced

Liebermann T, Knoll W (2003) Parallel multispot detection of target hybridization to surface-bound probe oligonucleotides of different base mismatch by surface-plasmon field-enhanced fluorescence microscopy. Langmuir 9 1567-1572... 

Alternatively, various analytical methods based on SPR phenomenon have been developed, including surface plasmon field-enhanced Raman scattering (SERS) [7], surface plasmon field-enhanced fluorescence spectroscopy (SPFS) [8-11], surface enhanced second harmonic generation (SHG) [12], surface enhanced infrared absorption (SEIRA) [13], surface plasmon field-enhanced diffraction spectroscopy (SPDS) [14-18], Most of these methods take advantage of the greatly enhanced electromagnetic field of surface plasmon waves, in order to excite a chromophoric molecule, e.g., a Raman molecule or a fluorescent dye. Therefore, a better sensitivity is expected. 

Surface Plasmon Field-Enhanced Fluorescence Spectroscopy... 

Fig. 13. (A) Schematic representation of the interaction between neighboring residues of the His-tag (6 consecutive Histidine residues) and an NTA-complexed Ni2+ ion. (B) The performance of LHCII immobilization via chelating interaction. SPR kinetic curve of LHCII immobilization and regeneration cycles, monitored with an Nd YAG DPSS laser (X = 473 nm). (C) Surface plasmon field-enhanced fluorescence emission spectrum of surface attached LHCII compared with the fluorescence emission from free LHCII in solution, excited by an Nd YAG DPSS laser (X — 473 nm).

Fig. 16. Photographs of the surface plasmon field-enhanced diffraction patterns generated by various PS patterns on Au surfaces (A) parallel lines 40 pm wide, separated by 62.5 pm (B) parallel lines 28 pm wide, separated by 83 pm (C) square grid pattern, bar width 28 pm, grid width 83 pm, [D] hexagonal grid pattern, bar width 8 pm, hexagonal grid width 48 pm.

Liebermann, T., and Knoll, W. (2000). Surface-plasmon field-enhanced fluorescence spectroscopy. Colloid and Surfaces A. Physicochemical and Engineering Aspects 171 115-130. 

Ekgasit, S., Thammacharoen, C., Yu, F., and Knoll, W. (2004). Evanescent Field in Surface Plasmon Resonance and Surface Plasmon Field-Enhanced Fluorescence Spectroscopies./Ina/. Chem. 76 2210-2219. 

Fundamentals. Surface plasmons (SP) can be used to monitor optical properties of metal and semiconductor surfaces. For introductory overviews, see [969, 970]. Surface plasmon field-enhanced light scattering (SPFELS) is observed when an interface is illuminated by light under conditions stimulating surface plasmon excitation as reported [971]. 

Fig. 5.155. Cyclic voltammogram of polyaniline and surface plasmon field-enhanced light scattering with a solution of 0.5 M H2SO4, second scan, dE jdt — 20 mV-s based on data in [971]...

SPFELS Surface plasmon field enhanced light scattering... 

If one includes the employment of evanescent light waves in spectroscopic studies in the near-field area. Knoll and coworkers are among the first who reported on near-field Raman spectroscopy , though this approach was termed at that time (the early 1990s) as surface plasmon field-enhanced Raman spectroscopy and microscopy [104],... 

Liebermann, T., Knoll, W. Surface-plasmon field-enhanced fluorescrmce spectroscopy. Colloids Surf. A Physiochem. Eng. Asp 10, 115-130 (2000)... 

PRINCIPLES AND APPLICATIONS OF SURFACE-PLASMON FIELD-ENHANCED FLUORESCENCE... 

In an attempt to overcome this limit of detection we recently introduced surface plasmon field-enhanced fluorescence spectroscopy (SPFS) following an earlier report liy Attridge et al. The basic principle of this approach combines the excitation of a surface plasmon mode as an interfacial light source with the well-established detection schemes of fluorescence spectroscopy the resonantly excited surface plasmon waves excite chromophores that are attached to the analyte either chemically or by genetic engineering techniques. The emitted fluorescence photons are then monitored and analyzed in the usual way to give information about the behavior of the analyte itself. 

Figure 17. Schematic experimental setup for surface-plasmon and surface-plasmon field-enhanced fluorescence microscopy in the Kretschmann configuration.

Yu YM, Feng CL, Caminade AM, Majoral JP, Knoll W (2009) The detection of DNA hybridization on phosphorus dendrimer multilayer films by surface plasmon field enhanced-fluorescence spectroscopy. Langmuir 25 13680-13684... 

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