Surface-enhanced fluorescence , plasmonic engineering

The Role Of Plasmonic Engineering In Potential Surface-Enhanced Fluorescence... 

Liebermann, T., and Knoll, W. (2000). Surface-plasmon field-enhanced fluorescence spectroscopy. Colloid and Surfaces A. Physicochemical and Engineering Aspects 171 115-130. 

In an attempt to overcome this limit of detection we recently introduced surface plasmon field-enhanced fluorescence spectroscopy (SPFS) following an earlier report liy Attridge et al. The basic principle of this approach combines the excitation of a surface plasmon mode as an interfacial light source with the well-established detection schemes of fluorescence spectroscopy the resonantly excited surface plasmon waves excite chromophores that are attached to the analyte either chemically or by genetic engineering techniques. The emitted fluorescence photons are then monitored and analyzed in the usual way to give information about the behavior of the analyte itself. 

The two SERS bands of eosin at 292 and 500 cm" are developed in spectrum 1 on Fig. 2b. It indicates the major plasmon-dependent mechanism of fluorescence enhancement. Besides the plasmon enhancement, the interference effect between two silver surfaces is possible. We suggest that both these effects are responsible for an observable phenomenon. But more sensitive parameters for the secondary emission enhancement can be tuned through engineering of LP band position and optical density. The low and disadvantageous spectral overlap of LP and molecular absorption bands, as well as the silver deposition excess lead to the significant quenching of analyte fluorescence. 

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