Chromatography surface

Yang, Q. Lundahl, P., Binding of lysozyme on the surface of entrapped phosphati-dylserine-phosphatidylcholine vesicles and an example of high-performance lipid vesicle surface chromatography, J. Chromatogr. 512, 377-386 (1990). 

This is invariably referred to in various literature as open-column chromatography drop chromatography strip-chromatography spread-layer chromatography surface chromatography . 

Q Yang, M Wallsten, P Lundahl. Lipid-vesicle-surface chromatography. J Chromatogr 506 379-389, 1990. 

Some further methods depend upon the accurate measurement of mass. Others depend upon the stickability of molecules to various surfaces (chromatography) and yet others upon their ability to take up the energy from various parts of the radiant energy spectrum. 

As long ago as 1949, Meinhard and Hall [16] applied the method termed surface chromatography by them, for separation of mixtures of simple ferric and zinc salts. 

Ganshirt, H., and Malzacher, A. (1960). Separation of several vitamins of the B group and of vitamin C by surface chromatography. Naturwissenschaften 47 279-280. 

SAMs are generating attention for numerous potential uses ranging from chromatography [SO] to substrates for liquid crystal alignment [SI]. Most attention has been focused on future application as nonlinear optical devices [49] however, their use to control electron transfer at electrochemical surfaces has already been realized [S2], In addition, they provide ideal model surfaces for studies of protein adsorption [S3]. 

Chromatography is based upon the selective adsorption from solution on the active surface of certain finely divided solids. Closely related substances exhibit different powers of adsorption, so that separations, which are extremely difficult by ordinary chemical methods, may be effected by this means. When, for example, a solution of leaf pigments... 

A form of chromatography in which the stationary phase is immobilized on a flat surface. 

Analytical separations may be classified in three ways by the physical state of the mobile phase and stationary phase by the method of contact between the mobile phase and stationary phase or by the chemical or physical mechanism responsible for separating the sample s constituents. The mobile phase is usually a liquid or a gas, and the stationary phase, when present, is a solid or a liquid film coated on a solid surface. Chromatographic techniques are often named by listing the type of mobile phase, followed by the type of stationary phase. Thus, in gas-liquid chromatography the mobile phase is a gas and the stationary phase is a liquid. If only one phase is indicated, as in gas chromatography, it is assumed to be the mobile phase. 

The most widely used particulate support is diatomaceous earth, which is composed of the silica skeletons of diatoms. These particles are quite porous, with surface areas of 0.5-7.5 m /g, which provides ample contact between the mobile phase and stationary phase. When hydrolyzed, the surface of a diatomaceous earth contains silanol groups (-SiOH), providing active sites that absorb solute molecules in gas-solid chromatography. 

For simple FI, the substance to be mass measured is volatilized by heating it close to the emitter so that its vapor can condense onto the surface of the electrode. In this form, an FI source can be used with gas chromatography, the GC effluent being passed over the emitter. However, for nonvolatile and/or thermally labile substances, a different approach must be used. 

SFC/MS. supercritical fluid chromatography and mass spectrometry used as a combined technique SID. surface-induced dissociation (or decomposition)... 

Hydrophobic Interaction Chromatography. Hydrophobic interactions of solutes with a stationary phase result in thek adsorption on neutral or mildly hydrophobic stationary phases. The solutes are adsorbed at a high salt concentration, and then desorbed in order of increasing surface hydrophobicity, in a decreasing kosmotrope gradient. This characteristic follows the order of the lyotropic series for the anions ... 

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