Phosphatidylcholine vesicles

Discuss the effects on the lipid phase transition of pure dimyris-toyl phosphatidylcholine vesicles of added (a) divalent cations, (b) cholesterol, (c) distearoyl phosphatidylserine, (d) dioleoyl phosphatidylcholine, and (e) integral membrane proteins. 

Korgel, B. A. and Monbouquette, H. G. (1996). Synthesis of Size-Monodisperse CdS Nanocrystals Using Phosphatidylcholine Vesicles as True Reaction Compartments. /. Phys. Chem., 100, 346-351. 

Huang, C. H. (1969). Studies on phosphatidylcholine vesicles. Formation and physical characteristics, Biochemistry. 8, 344-352. 

Bhakuni V, Roy R. 1994. Interaction of trichloroethylene with phosphatidylcholine and its localization in the phosphatidylcholine vesicles A IH-NMR study. Biochem Pharmacol 47 1461-1464. 

Yang, Q. Lundahl, P., Binding of lysozyme on the surface of entrapped phosphati-dylserine-phosphatidylcholine vesicles and an example of high-performance lipid vesicle surface chromatography, J. Chromatogr. 512, 377-386 (1990). 

Yeagle, P. L. Hutton, W. C. Huang, C.-H. Martin, R. B., Headgroup conformation and lipid-cholesterol association in phosphatidylcholine vesicles A 31P 1H nuclear Over-hauser effect study, Proc. Natl. Acad. Sci. 72, 3477-3481 (1975). 

Norris, F. A. Powell, G. L., The apparent permeability coefficient forproton flux through phosphatidylcholine vesicles is dependent on the direction of flux, Biochim. Biophys. Acta 1030, 165-171 (1990). 

Lessard, J. G. Fragata, M., Micropolarities of lipid bilayers and micelles. 3. Effect of monovalent ions on the dielectric constant of the water-membrane interface of unilamellar phosphatidylcholine vesicles, J. Phys. Chem. 90, 811-817 (1986). 

Huang, C. Studies of Phosphatidylcholine Vesicles. Formation and Physical Characteristics, Biochemistry, 1969, 8, 344. 

Clarke RJ, Liipfert C (1999) Influence of anions and cations on the dipole potential of phosphatidylcholine vesicles a basis for the Hofmeister effect. Biophys J 76 2614-2624... 

The 2-anthroyl fluorophore can be incorporated synthetically in phosphatidylcholine vesicles (anthroyl-PC), which provides an elegant tool for investigating the bilayers of egg phosphatidylcholine vesicles (Perochon et al., 1992). 

Plots of t0/t for varying [Q]T are first otained for different concentrations of lipid. Then, form the slope and intercept of a plot of k pp against am, the value of the partition coefficient is obtained. This method has been applied to the partitioning of lindane into lipid bilayers.(102 103) An example is shown in Figure 5.4 for the quenching of DPH by 5-DOXYL-decane in dimyristoyl-phosphatidylcholine vesicles(104) the increase in the partition coefficient as the... 

Figure 5.4. EfTect of the phase transition on the partition coefficient of 5-DOXYL-decane calculated on the basis of its ability to quench the fluorescence of DPH in dimyristoyl-phosphatidylcholine vesicles. The fluorescence anisotropy of the DPH is also shown. (From Ref. 104, with permission.)...

M. Straume and B. J. Litman, Equilibrium and dynamic structure of large, unilamellar, unsaturated acyl chain phosphatidylcholine vesicles. Higher order analysis of 1,6-diphenyl-... 

P. L.-G. Chong and T. E. Thompson, Oxygen quenching of pyrene-lipid fluorescence in phosphatidylcholine vesicles, Biophys. J. 47, 613-621 (1985). 

Maeda M, Kumano A, TirteU DA. H -I- -Induced release of contents of phosphatidylcholine vesicles bearing surface-bound poly-electrolyte chains. J Am Chem Soc 1988 110 7455-7459. 

Single-compartment egg yolk phosphatidylcholine vesicles Au Nanosized Au particles precipitated in the vesicles 78... 

The synthesis of the photoaffinity probe, NAZA-FL, lc, will be described elsewhere. The effects of this acyclic modifier on mitochondrial activities were assayed as previously described (5,6). The results are summarized in the Table. The effects, and the effective concentration range, of compounds lc and La are similar in the dark. With the photolabile modifier,< T(c, illumination with strong light resulted in an irreversible preferential inhibition of the energy-dependent proton movements this effect was not relieved by phosphatidylcholine vesicles. Hence, the modifier became covalently bonded in the vicinity of its implantation. Additional experiments were performed with phosphate-washed (12) membrane preparations (ATPase-enriched inner membrane frac-... 

Bertoli, E., Masserini, M., Sonnino, S., Ghidoni, R., Cestaro, B., and Tettamanti, G. (1981), Electron paramagnetic resonance studies on the fluidity and surface dynamics of egg phosphatidylcholine vesicles containing gangliosides, Biochim. Biophys. Acta, 647, 196-202. 

Unilamellar vesicles are usually formed from lipid dispersions with sonication. Thus the suspension is metastablc, and vesicles aggregate to form a multilamellar structure. The decay time of sonicated phosphatidylcholine vesicles, as measured by DLS, showed a bimodal distribution 50. The hydrodynamic radius estimated from the smaller decay time was consistent with the values obtained by other experimental techniques such as ultracentrifugation. 

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