Sulfonyl chloride Lissamine rhodamine

Estradiol 5-Dimethylaminonaphthalene-l -sulfonyl chloride Lissamine rhodamine B sulfonyl chloride 87... 

Laryl chloride. Lissamine rhodamine B sulfonyl chloride [62796-29-6]... 

Remove unreacted NHS-rhodamine and reaction by-products by gel filtration or dialysis. Lissamine Rhodamine B Sulfonyl Chloride... 

Figure 9.16 Lissamine rhodamine B sulfonyl chloride reacts with amine-containing molecules to produce stable sulfonamide bonds.

Lissamine rhodamine B sulfonyl chloride is relatively insoluble in water, but may be dissolved in DMF prior to the addition of a small aliquot to an aqueous reaction. Do not dissolve in DMSO, as sulfonyl chlorides will readily react with this solvent (Boyle, 1966). The compound has a maximal absorptivity at 556 nm with an extremely high extinction coefficient of up to 93,000M em-1 (in methanol) in highly purified form. Its emission maximum occurs at 576 nm, emitting red luminescence. 

Lissamine rhodamine B sulfonyl chloride has been used in numerous applications, including multiple-labeling techniques in microscopy (Wessendorf, 1990), for confocal microscopy... 

The following protocol is a general guide for labeling biological macromolecules with Lissamine rhodamine B sulfonyl chloride. Optimization of the fluorophore incorporation level (F/P ratio) may have to be done for specific labeling experiments. 

Texas Red hydrazide is a derivative of Texas Red sulfonyl chloride made by reaction with hydrazine (Invitrogen). The result is a sulfonyl hydrazine group on the No. 5 carbon position of the lower-ring structure of sulforhodamine 101. The intense Texas Red fluorophore has a QY that is inherently higher than either the tetramethylrhodamine or Lissamine rhodamine B derivatives of the basic rhodamine molecule. Texas Red s luminescence is shifted maximally into the red region of the spectrum, and its emission peak only minimally overlaps with that of fluorescein. This makes derivatives of this fluorescent probe among the best choices of labels for use in double-staining techniques. 

Modification with Lissamine Rhodamine B Sulfonyl Chloride... 

Dissolve Lissamine rhodamine B sulfonyl chloride in DMF at a concentration of 1-2 mg/ml. Protect from light and use immediately. Do not use DMSO as the solvent, as sulfonyl chlorides react with it. 

Figure 23.7 Avidin (or (strept)avidin) can be labeled with Lissamine rhodamine sulfonyl chloride to form a fluorescent probe.

Lissamin rhodamine B sulfonyl chloride chemiluminescence reagent, 5 852-853 Lister, Joseph, 11 7 Literature research, in fine chemical... 

The red-emitting rhodamine derivatives are constructed around the same basic xanthene framework as is fluorescein (2). Tetramethylrhodamine isothiocyanate (TRITC) has been widely employed for immunofluorescence. Additional derivatives of rhodamine available for conjugation to antibodies include lissamine rhodamine sulfonyl chloride (RB-200-SC), rhodamine B isothiocyanate (RBITC), rhodamine X isothiocyanate (XRITC), and Texas... 

This approach has been shown to work with a number of different fluorescent probes such as the short-wavelength fluorophores dansyl sul-fonyl chloride and coumarin chloride and the long-wavelength fluorophores tetramethylrhodamine-5-(and-6)-isothiocyanate [5(6)-TRITC], 5-(and-6)-carboxytetramethylrhodamine, succinimidyl ester [5(6)-TAMRA, succin-imidyl ester] and lissamine rhodamine B sulfonyl chloride (each in conjunction with different binding functionalities on the SAM surface. 

The red-emitting rhodamine derivatives are constructed around the same basic xanthene framework as is fluorescein (2). Tetramethyl-rhodamine isothiocyanate (TRITC) has been widely employed for immunofluorescence. Additional derivatives of rhodamine available for conjugation to antibodies include lissamine rhodamine sulfonyl chloride (RB-200-SC), rhodamine B isothiocyanate (RBITC), rhodamine X isothiocyanate (XRITC), and Texas Red (Molecular Probes, Inc.). The spectra of XRITC and Texas Red are shifted to longer wavelengths compared to those of other rhodamines, which makes them particularly useful for combination with fluorescein in dual-labeling procedures see Section 5, below). Of the two, Texas Red, which is more hydrophilic and less likely to precipitate proteins upon conjugation (12), is more commonly employed. 

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