Dual-label

Not detected High levels Dual-label in situ hybridization Hrabovszky et al. 2000... 

Up to 50 stored assay protocols include routines for RIA/IRMA, dual label assays, T3-uptake and FTI calculations and hepatitis, RAST and hCG screening. Quality-control charts can be printed automatically. 

Z -LYTE Invitrogen Phosphorylation by a kinase of dual labeled FRET peptide changes its susceptibility to proteolytic cleavage... 

Photons with detectable energy differences that are emitted by various radionuclides can be quantified simultaneously, but independently from each other. This allows the use of dual-labeling approaches (4). These experiments will reveal information regarding both the liposomal carrier—labeled with one radionuclide—and the encapsulated compound—labeled with a different radionuclide—after a single injection in the same animal. However,... 

Red. The spectra of XRITC and Texas Red are shifted to longer wavelengths compared to those of other rhodamines, which makes them particularly useful for dual labeling procedures in comhination with fluorescein (see Subheading 5.). Of the two, Texas Red, which is more hydrophilic and less likely to precipitate proteins upon conjugation (12), is more commonly employed. 

P.Z. Tan, R.M. Baldwin, T. Fu, D.S. Charney, R.B. Innis, Rapid synthesis of F-18 and H-2 dual-labeled altanserin, a metabolically resistant PET ligand for 5-HT2A receptors, J. Label. Compds Radiopharm. 42 (1999) 457-467. 

Ge XD, Tolosa L, Rao G. Dual-labeled glucose binding protein for ratiometric measurements of glucose. Analytical Chemistry 2004, 76, 1403-1410. 

Key words Cholesterol absorption, phytosterols, cholesterol excretion, reverse cholesterol transport, ACAT, CEL, inhibitors, bile acids, fecal sterols, dual label, obesity, cardiovascular disease. 

Transfer 0.5 ml of the combined extract to a fresh scintillation vial, evaporate the solvent, resuspend the residue in 0.25 ml methanol or ethanol, add scintillation cocktail, and count using a 3H, 14C dual-label program. 

Figure 6.2 Biosynthesis of cucujolide I from ( , )-farnesol. Oxidative cleavage of the terminal double bond would yield a hydroxy acid derivative, which could cyclize to give cucujolide I (X = a leaving group such as CoA). Dual labeling experiments with 2H and 180 indicated that the hydroxyl oxygen of farnesol is retained in the product (Vanderwel et al., 1992b).

Fig. 12. Dual-label time-resolved fluoroimmunoassay of AFP and CEA with luminescent lanthanide labels BHHCT-...

Fig. 4.2.8. Homogeneous DNA detection by employing fluorescence resonance energy transfer between two labels. Dual label interaction between (a) dyes that bind to dsDNA...

B21. Brugge, W. R., Goff, J. S., Allen, N. C., Podell, E. R., and Allen, R. H., Development of a dual label Schilling test for pancreatic exocrine function based on the differential absorption of cobalamin bound to intrinsic factor and R protein. Gastroenterology 78, 937-949 (1980). 

These studies demonstrate that treatments that have been reported to modify the heat stability of milk also may change the distribution of tracer milk proteins between the physical phases of milk. Analysis of these interactions may provide useful information about the mechanisms of these effects. In unpublished experiments, we have extended this approach for studying homogenized milk-based systems, also containing a lipid phase, to investigate lipid-protein interactions. Dual-label experiments, for example in milk containing M-/3-L and 3H-methyl-/c-casein, could be applied to the isolation and characterization of protein complexes in milk. Labeled caseins could prove valuable as probes for elucidating micelle structure. 

Drug code labels come in many forms. One of the most reliable is the dual-labeled form, in which the code is covered by a mercury film that can be scratched off easily by a coin or similar blunt object to reveal the identity of the contents. Another effective type is the three-sided envelope A B C in front and C B A in back. Section C contains the written code and is sealed to part B. If an emergency arises, the detached code label portion is immersed in warm... 

Based upon Eq. 4 a systematic study was performed with four polar permeants (urea, mannitol, sucrose, and raffinose) in an effort to characterize further the porous permeation pathway through HEM (Peck et al., 1994). Dual-labeled liquid scintillation counting and an experimental protocol that incorporated successive permeability experiments, as outlined in the previous sections, allowed the permeability coefficients for each permeant to be determined for each HEM sample studied. Again, Eq. 4 predicts that, for a porous membrane, the permeability coefficient ratio should be equal to the ratio of the diffusion coefficients for the solutes in the membrane. As a first approximation, if the relative radii of the solutes and the membrane pore radii Rp are such that hindrance considerations are negligible (Deen, 1987), then the ratio PJPy should approach the ratio of the free diffusion coefficients D of the solutes in bulk solution. 

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