Lissamine rhodamine B sulfonyl chloride

Modification with Lissamine Rhodamine B Sulfonyl Chloride 

Dissolve (strept)avidin in 0.1 M sodium carbonate/bicarbonate buffer, pH 9.0, at a concentration of 1-5 mg/ml. 

Dissolve Lissamine rhodamine B sulfonyl chloride in DMF at a concentration of 1-2 mg/ml. Protect from light and use immediately. Do not use DMSO as the solvent, as sulfonyl chlorides react with it. 

In a darkened lab and with gentle mixing, slowly add 50-100 pi of the fluorophore solution to each ml of the (strept)avidin solution. 

Remove excess fluorophore by gel filtration using a column of Sephadex G-25 or by dialysis. 

---

Remove unreacted NHS-rhodamine and reaction by-products by gel filtration or dialysis. Lissamine Rhodamine B Sulfonyl Chloride... 

Figure 9.16 Lissamine rhodamine B sulfonyl chloride reacts with amine-containing molecules to produce stable sulfonamide bonds.

Lissamine rhodamine B sulfonyl chloride is relatively insoluble in water, but may be dissolved in DMF prior to the addition of a small aliquot to an aqueous reaction. Do not dissolve in DMSO, as sulfonyl chlorides will readily react with this solvent (Boyle, 1966). The compound has a maximal absorptivity at 556 nm with an extremely high extinction coefficient of up to 93,000M em-1 (in methanol) in highly purified form. Its emission maximum occurs at 576 nm, emitting red luminescence. 

Lissamine rhodamine B sulfonyl chloride has been used in numerous applications, including multiple-labeling techniques in microscopy (Wessendorf, 1990), for confocal microscopy... 

The following protocol is a general guide for labeling biological macromolecules with Lissamine rhodamine B sulfonyl chloride. Optimization of the fluorophore incorporation level (F/P ratio) may have to be done for specific labeling experiments. 

Estradiol 5-Dimethylaminonaphthalene-l -sulfonyl chloride Lissamine rhodamine B sulfonyl chloride 87... 

Lissamin rhodamine B sulfonyl chloride chemiluminescence reagent, 5 852-853 Lister, Joseph, 11 7 Literature research, in fine chemical... 

Fluorescein isothiocyanate (FITC) or Lissamine rhodamine B sulfonyl chloride (RBSC). 

This approach has been shown to work with a number of different fluorescent probes such as the short-wavelength fluorophores dansyl sul-fonyl chloride and coumarin chloride and the long-wavelength fluorophores tetramethylrhodamine-5-(and-6)-isothiocyanate [5(6)-TRITC], 5-(and-6)-carboxytetramethylrhodamine, succinimidyl ester [5(6)-TAMRA, succin-imidyl ester] and lissamine rhodamine B sulfonyl chloride (each in conjunction with different binding functionalities on the SAM surface. 

Linocaffein, in D-00716 Lissamine blue BF, L-00007 Lissamine red W, see A-00477 Lissamine rhodamine B sulfonyl chloride, see C-00261... 

---