Sugar analyses solution

Polarization is the most common method for the determination of sugar in sugar-containing commodities as well as many foodstuffs. Polarimetry is apphed in sugar analysis based on the fact that the optical rotation of pure sucrose solutions is a linear function of the sucrose concentration of the solution. Saccharimeters are polarimeters in which the scales have been modified to read directiy in percent sucrose based on the normal sugar solution reading 100%. 

Phosphorus, fatty acids, carbohydrates, glycerol, and amino acids were analyzed by the method described in our previous paper [8] and references cited therein. SDS-PAGE [8], TLC [9], HPLC [9], determination of phos-phomonoester [8], reducing sugar analysis [13], methylation analysis [14], and hexose analysis [15] were performed as described in the respective literature. Two dimensional TLC was performed on silica-gel plate (Merck Silicagel 60 F254 No. 5715) using the solvent systems, chloroform-methanol-acetic acid (65/10/1, v/v/v) for the first development and chloroform-methanol-25% ammonia solution (65/10/1) for the second. 

These basic acetates are used in the manufacture of pigments in dyeing and printing fabrics, the aqueous solutions in medicine for washes and poultices in sugar analysis and as starting materials for preparing other lead salts. 

Fehlmg s solution -in sugar analysis [SUGAR- SUGARANALYSIS] (Vol 23)... 

Polan2ation magnetic [MAGNETIC MATERIALS - BULK] (Vol 15) measurements by scattering techniques [SPECTROSCOPY, OPTICAL] (Vol 22) of sugar solutions [SUGAR - SUGAR ANALYSIS] (Vol 23)... 

Tnformation about the characteristics of keto-hexoses in solution has been - derived mainly from optical rotatory data (I, 2, 3, 4) and in recent years by application of the principles of conformational analysis (5, 6). In the current study an attempt is made to describe the conformation and composition of these sugars in solution by nuclear magnetic resonance (NMR) spectroscopy, a highly sensitive means for examining stereochemistry and for differentiating between isomeric species. 

As a result of decisions of International Congresses of Applied Chemistry, among them that held at Paris (1900) and of the International Commission for Standard Methods of Sugar Analysis, the specific gravity of saccharine solutions should be determined at 20° C. and referred to water at 40 (sp. gr. at 20°/4°), i.e., it should indicate the weight of a true c.c. of solution at 200. Use is, however, largely made in practice of the sp. gr. at i745°/i7-50 and sometimes at i5°/i5°. 

The refractive index of a pure sucrose solution is an accurate measure of the concentration of dissolved substance. Stolle (39) found little variation in the refractive index of solutions of different sugars (sucrose, dextrose, levulose and lactose) at the same concentration. Investigations by Main (40), Schonrock (41) and Landt (42) resulted in the adoption of a standard table of refractive indices of sugar solutions by the International Commission for Uniform Methods of Sugar Analysis in 1936. 

Because of rapid, reversible reactions, direct chemical methods for measuring the concentration of the acyclic form of a sugar in solution are not generally satisfactory. Attempts have been made to estimate the proportion of the acyclic form by study of the reactions of sugEirs with chlorous acid and with cyanide. The results clearly show the absence of a large concentration of the acyclic modification in the solution, but the methods do not give satisfactory values for the concentration, because the rate of formation of the acyclic modification is fast in comparison with the time required for the analysis. 

P8 >20 Coarse 3.0 Gelatinous precipitates, clarifying solutions, oils, sugar analysis... 

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