Saccharin solution

For example, if saccharin (33) is methylated in benzene suspension, then only iV-methylsaccharin is isolated. If an ethereal saccharin solution is added to a concentrated solution of diazomethane in excess, then 10% of 0-methylsaecharin (34) can be detected in addition to the A"-raethy] derivative. Finally, if the diazomethane solution is gradually added to a saturated ethereal solution of saccharin, the proportion of 0-methylation increases to 24%. " ... 

No deaths in any group. The 30 mg/kg group was hyperactive for 4 h after dosing. All mice preferred 0.3% saccharin solution to water... 

Determination of the Specific Gravity.—Tlic specific gravity of saccharine solutions is determined, at a certain normal temperature, by... 

As a result of decisions of International Congresses of Applied Chemistry, among them that held at Paris (1900) and of the International Commission for Standard Methods of Sugar Analysis, the specific gravity of saccharine solutions should be determined at 20° C. and referred to water at 40 (sp. gr. at 20°/4°), i.e., it should indicate the weight of a true c.c. of solution at 200. Use is, however, largely made in practice of the sp. gr. at i745°/i7-50 and sometimes at i5°/i5°. 

Saccharimeters.—For saccharine solutions use is made of polari-meters with a special graduation, these being termed saccharimeters. 

Girlich [1.40] studied by NMR the molecular dynamics of aqueous saccharin solutions. At concentrations down to 30% solids, the saccharin molecules do not influence each other, while with decreasing temperature the existing H-bond bridges prevent a reorientation of the H20 molecules. Dissolved saccharin molecules can destroy... 

Riley et al. (ref. 157) have shown that 10 and 15 day-old offspring of rats treated on gestational days 6-20 with ethanol ingest less of a saccharin solution paired previously with LiCl than controls, although they show a clear taste aversion. They interpret this result as additional evidence for a deficient passive avoidance learning. A decreased passive avoidance performance using the more traditional shock punishment has also been shovn in rats prenatally treated with ethanol. However, pre-exposure to unpleasant... 

The behavioral effects of dermal exposure to maneb were studied in adult male Swiss mice (Mitchell et al. 1989). Five mice per dose group were dermally exposed to either 16, 160, or 1,600 mg/kg maneb in a conditioned taste aversion assay. Aversion to a saccharin solution was tested 24 hours following the dermal application of the compound to a shaved area on the mouse s back, immediately caudal to the skull. Maneb application did not affect taste aversion compared to water controls. Activity was measured for both ambulatory and nonambulatory activity ambulatory was when a subject sequentially disrupted at least two adjacent infrared beams in an activity monitor nonambulatory activity was when a subject sequentially broke the same beam. When given at a dose of 1,600 mg/kg, maneb increased the activity levels of a tested group (5 subjects) by 3-fold over controls. When the mice were tested individually, however, there were no... 

Similarly, with the sense of taste, most animals show preferences for certain foods when presented with a choice. Typical is the preference of yoimg pigs for sucrose solutions rather than water. The fowl is indifferent to solutions of the common sugars but finds xylose objectionable, and it will not ingest salt solutions in concentrations beyond the capacity of its excretory system. Every species studied has shown considerable individual variability for example, in a litter of pigs tested with saccharin solutions of different concentrations, some animals preferred high levels of the sweetener whereas others rejected them. 

Note to paragraph 3. (a) If the test subject eats or drinks something sweet before the screening test, he/she may be unable to taste the weak saccharin solution. 

Gao et al. [91] reported a FIA chemiluminescence determination of sodium saccharin based on the chemiluminescent reaction between luminol and hydrogen peroxide. In an acid medium, the sodium saccharin solution was reacted with potassium dichromate to reduce Cr(VI) to Cr(III), and the chemiluminescence intensity of the reaction was remarkably enhanced by the Cr(III) formed. The content of Cr(III) was then determined and converted into contents of sodium saccharin. 

Naphthalin Nickel Chloride, nitrate or R Saccharin solutions Salicylic acid R 32... 

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