Sudan II

Fig. 3.38.The IUPAC names of Sudan azo dyes are as follows Sudan 1 = 1— [(2,4-dimethylphenyl)azo]-2-naphtalenol Sudan II = l-(phenylazo)-2-naphtol Sudan III = l-(4-phenylazophenylazo)-2-naphtol Sudan IV = o-tolyazo-o-tolyazo-beta-naphtol and Disperse Orange 13 = 4-[4-(phenylazo)-l-naphtylazo]-phenol. Azo dyes were separated in an ODS column (250 x 2.1 mm i.d. particle size 5 /xm) at 35°C. The isocratic mobile phase consisted of 0.1 per cent formic acid in methanol-0.1 per cent formic acid in water (97 3, v/v). The flow rate was 200 /xl/min. MS conditions were nebulizing and desolvation gas were nitrogen at the flow rates of 50 and 5551/h, respectively electrospray voltage, 3.0 kV cone voltage 25 V source temperature, 110°C desolvation temperature, 110°C. Azo dyes were extracted from the samples by homogenizing 1 g of sample with 10 ml of acetone, then the suspension was centrifuged and an aliquot of 3 ml of supernatant was mixed with 1 ml of deionized water, filtered and used for analysis. LC-ESI-MS/Ms SRM traces of standards and spiked samples are listed in Fig. 3.39. It was found that the detection and quantitation limits depended on both the chemical structure of the dye and the character of the accompanying matrix. LOD and LOQ values in chilli tomato sauce...

Fig. 3.39. LC-ESI-MS/MS SRM traces obtained from 125 pgfi standard solution of (a) Sudan I (b) Sudan II (c) Sudan IE (d) Sudan IV (e) Disperse orange 13 internal standard (100 /zg/1 left column) and from a blank chilli tomato and cheese sauce sample spiked with 125 pgfl each (1 685 jug/kg sample) of (f) Sudan I (g) Sudan II (h) Sudan III (i) Sudan IV (j) Disperse orange 13 internal standard (100 /ig/1 right column injection volume 20 p). Reprinted with permission from F. Calbiani et al. [115].

F. Calbiani, M. Careri, L. Elviri, A. Mangia, L. Pistara and I. Zagnoni, Development and in-house validation of a liquid chromatography-electrospray-tandem mass spectrometry method for the simultaneous determination of Sudan I, Sudan II, Sudan II and Sudan IV in hot chili products. J. ChromatogrA, 1043 (2004) 123-130. 

Succinic anhydride Sudan I Sudan II Sudan III Sudan Brown RR Sudan Red 7B Sulfafurazole... 

Sakaeda,T.,Takahashi, K., Nishihara, Y., and Hirano, K. (1994), OAV lipid emulsions for parenteral drug delivery. I. Pharmacokinetics of the oil particles and incorporated sudan II, Biol. Pharm. Bull, 17,1490-1495. 

The use of saturated MCT was the most effective way to increase blotxl concentration of Sudan II. resulting in higher distribution to liver, lungs, spleen, and brain (52). 

Sudan II (Sudan Blue, Solvent Blue 35, l,4-bis-(butylamino)-9,10-anthraquinone) [17354-14-2] M 350.5, m 122°, Xmax 604, 652nm, pKj st 9.5 (OH). It is formed from quinizarin (2g see [81-64-1]), 33% EtOH/w-BuNH2 (20ml) and Na2S204 (2g) at 140°/8 hours, evaporate, extract with toluene, chromatograph (AI2O3), the intense blue band in toluene is evaporated, and the residue gave purple needles (Cu lustre) from petroleum ether (b 60-80°) (l.lg, 38%) [Peters Walker JC/zewz Soc 1429 1956, Beilstein 14 IV 460], It forms Cu and Ni salts. 

Sudan I, Sudan II, Sudan Red B, Sudan Orange G Sudan III, Sudan IV, Sudan Red 7B, Para Red) Water-soluble food dyes... 

After the twofold development (HTpSPE), the target zones—visible by Sudan II—were easily eluted by the TLC-MS interface into autosampler vials to perform the offline HTpSPE-HPLC-MS(/MS) analysis. The direct transfer of the eluates by the online HTpSPE-HPLC-MS(/MS) hyphenation was additionally possible, but due to the poor time efficiency not routinely applied. The cleanup effect of HTpSPE, especially attributed to the separation of caffeine, was easily demonstrated by HPLC-MS analysis in the full-scan mode (Eigure 10.13). 

QuEChERS-dSPE extracts (A), acetonitrile-dSPE extracts before (B) and after HTpSPE (C) (a) blank extracts, (b) extracts spiked with pesticides at 1 mg/kg and TDCPP ( ), Sudan II (s). (Reprinted from Oellig, C., and Schwack, W., J. Chromatogr. A, 1260, 42-53, 2012. Copyright 2012, with permission from Elsevier.)... 

Chailapakul, O., Wonsawat, W Siangproh, W., Grudpan, K., Zhao, Y and Zhu, Z. (2008) Analysis of Sudan I, Sudan II, Sudan III, and Sudan IV in food by HPLC with electrochemical detection comparison of glassy carbon electrode with carbon nanotube-ionic liquid gel modifled electrode. Food Chem., 109 (4), 876-882. 

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