Structure-based engineering

Keywords Chalcone synthase superfamily enzyme, Engineered biosynthesis, Precursor-directed biosynthesis, Structure-based engineering, Type III polyketide synthase... 

Yang J, Fu X, Liao J et al (2005) Structure-based engineering of E. coli galactokinase as a first step toward in vivo glycorandomization. Chem Biol 12 657-664... 

Hoffmeister D, Thorson JS (2004) Mechanistic implications of Escherichia coli galactokinase structure-based engineering. ChemBioChem 5 989-992... 

Miller MA, Geren L, Han GW, et al. Identifying the physiological electron transfer site of cytochrome c peroxidase by structure-based engineering. Biochemistry 1996 35 667-73. 

Diacovich L, Mitchell DL, Pham H, Gago G, Melgar MM, 27. Khosla C, Gramajo H, Tsai S-C. Crystal structure of the ji-subunit of acyl-coa carboxylase structure-based engineering of substrate specificity. Biochemistry 2004 43 14027-14036. 28. 

Loris EA et al (2007) Structure-based engineering of strictosidine synthase auxiliary for alkaloid libraries. Chem Biol 14 979-985... 

Pego AP, Siebum SB, Luyn MJAV, et al. Preparation of degradable porous structures based on 1,3-trimethylene carbonate and D,L-lactide(co)polymers for heart tissue engineering. Tissue Eng, 2003, 9, 981 994. 

With the idea of extending the scope of the macromolecular engineering of aliphatic polyesters, the coordination-insertion ROP of lactones and dilactones has been combined with other polymerization processes. This section aims at reviewing the new synthetic routes developed during the last few years for building up novel (co)polymer structures based on aliphatic polyesters, at least partially. 

Henry, C. 2001. Structure-based drug design. Chemical and Engineering News (June 4) 69-78. 

Genetic engineering. The X-ray structures are known for many hydrolases, allowing for modeling of the substrate in the active site as well as structurally based, random or rational protein mutation to magnify or invert enantioselectivity. An example of the latter is provided by the rational design of a mutant of Candida antarctica lipase (CALB), which, instead of the wild-type R-selectivity, displayed... 

While the interaction of the substrate (and the inhibitor) with the catalytic zinc is the most important interaction, the remainder of the substrate (inhibitor) also forms hydrogen bonds with residues from the top strand of the 3 sheet and the loop region posterior to the Met-tum. These interactions with the substrate in the binding pockets of the MMPs are the prime targets for engineering specific MMP inhibitors. An in-depth understanding of the differences of the properties of these pockets in the different MMPs and the interactions of specific residues within these pockets is essential for structure-based design of inhibitors. 

Silicon carbide fibers have been used with aluminum, titanium, and coball-based superalloys for high-temperature structures and engine components. 

Engineered Biosynthesis of Plant Polyketides Structure-Based and Precursor-Directed Approach... 

Barton WA, Biggins JB, Jiang J et al (2002) Expanding pyrimidine diphosphosugar libraries via structure-based nucleotidylyltransferase engineering. Proc Natl Acad Sci USA 99 13397-13402... 

Thorson JS, William AB, Dirk H et al (2004) Structure-based enzyme engineering and its impact on in vitro glycorandomization. ChemBioChem 5 16-25... 

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